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Identification of Multiple Domains of Entamoeba histolytica Intermediate Subunit Lectin-1 with Hemolytic and Cytotoxic Activities
http://hdl.handle.net/10069/00041663
http://hdl.handle.net/10069/00041663610b0984-b7db-4831-9e1c-1dde9c106a2a
名前 / ファイル | ライセンス | アクション |
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IJMS23_7700.pdf (4.8 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2022-08-08 | |||||
タイトル | ||||||
タイトル | Identification of Multiple Domains of Entamoeba histolytica Intermediate Subunit Lectin-1 with Hemolytic and Cytotoxic Activities | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Entamoeba histolytica | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | intermediate subunit lectin-1 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | galactose/N-acetylgalactosamine (GalNAc)-inhibitable lectin | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | hemolytic activity | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | cytotoxicity | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Kato, Kentaro
× Kato, Kentaro× Tachibana, Hiroshi |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Galactose and N-acetyl-D-galactosamine-inhibitable lectin of Entamoeba histolytica have roles in the pathogenicity of intestinal amoebiasis. Igl1, the intermediate subunit lectin-1 of E. histolytica, has been shown to have both hemolytic and cytotoxic activities that reside in the C-terminus of the protein. To identify the amino acid regions responsible for these activities, recombinant proteins were prepared and used in hemolytic and cytotoxic assays. The results revealed that Igl1 has multiple domains with hemolytic and cytotoxic activities and that amino acids 787-846, 968-1028 and 1029-1088 are involved in these activities. The hemolytic activities of the fragments were partly inhibited by mannose, galactose and N-acetylgalactosamine, and glucose showed lower or negligible inhibitory effects for the activities. This is the first report of a protozoan protein with hemolytic and cytotoxic activities in multiple domains. | |||||
書誌情報 |
International Journal of Molecular Sciences 巻 23, 号 14, p. 7700, 発行日 2022-07-12 |
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出版者 | ||||||
出版者 | MDPI | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1422-0067 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.3390/ijms23147700 | |||||
権利 | ||||||
権利情報 | © 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/ | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | International Journal of Molecular Sciences, 23 (14), art. no. 7700; 2022 |