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Application of droplet digital PCR in the analysis of genome integration and organization of the transgene in BAC transgenic mice
http://hdl.handle.net/10069/38216
http://hdl.handle.net/10069/38216266704c0-b004-4f41-ab67-7e8dd6c5c9ae
名前 / ファイル | ライセンス | アクション |
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SciRep8_6638.pdf (2.1 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2018-05-25 | |||||
タイトル | ||||||
タイトル | Application of droplet digital PCR in the analysis of genome integration and organization of the transgene in BAC transgenic mice | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Nakagaki, Ayumi
× Nakagaki, Ayumi× Urakawa, Asuka× Hirano, Shiori× Anami, Takeru× Kishino, Tatsuya |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Transgenic (Tg) mice containing bacterial artificial chromosome (BAC) DNA are widely used for gene expression analysis and gene therapy models because BAC transgenes provide gene expression at physiological levels with the same developmental timing as endogenous genes. To ensure correct interpretation of transgene functions, investigation of the genomic organisation and integration of the BAC transgene is required. Here, we describe a reliable method based on droplet digital PCR (ddPCR) and inverse PCR to estimate copy number, genomic organisation and insertion sites of BAC transgenes in the mouse genome. We generated BAC Tg mice containing fragments of BAC clone RP23-59P20. ddPCR and iPCR analysis showed that the transgene consisted of five fragments of the BAC clone containing the Mkrn3 gene region, and that the transgene was inserted into Bckdhb, homozygous deletion of which causes the maple syrup urine disease phenotype. The ddPCR method described here should prove useful for analysis of genomic organisation and integration of BAC transgenes. | |||||
書誌情報 |
Scientific Reports 巻 8, p. 6638, 発行日 2018-04-27 |
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出版者 | ||||||
出版者 | Nature Publishing Group | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 2045-2322 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1038/s41598-018-25001-x | |||||
権利 | ||||||
権利情報 | c 2018 The Author(s). | |||||
権利 | ||||||
権利情報 | This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Scientific Reports, 8, 6638; 2018 |