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Accuracy of High-Throughput Nanofluidic PCR-Based Pneumococcal Serotyping and Quantification Assays Using Sputum Samples for Diagnosing Vaccine Serotype Pneumococcal Pneumonia: Analyses by Composite Diagnostic Standards and Bayesian Latent Class Models
http://hdl.handle.net/10069/38217
http://hdl.handle.net/10069/3821741f76bd8-8a2c-418c-8321-5dbd47db4a7e
名前 / ファイル | ライセンス | アクション |
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JCM56_1874-17.pdf (196.7 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2018-05-28 | |||||
タイトル | ||||||
タイトル | Accuracy of High-Throughput Nanofluidic PCR-Based Pneumococcal Serotyping and Quantification Assays Using Sputum Samples for Diagnosing Vaccine Serotype Pneumococcal Pneumonia: Analyses by Composite Diagnostic Standards and Bayesian Latent Class Models | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Bayesian latent class models | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Streptococcus pneumoniae | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | adult pneumonia | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | sputum culture | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | sputum real-time PCR | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | urinary antigen detection | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Kakiuchi, Satoshi
× Kakiuchi, Satoshi× Suzuki, Motoi× Dhoubhadel, Bhim Gopal× Furumoto, Akitsugu× Ito, Hiroyuki× Matsuki, Kei× Tsuchihashi, Yoshiko× Asoh, Norichika× Yasunami, Michio× Ariyoshi, Koya× Morimoto, Konosuke |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | The lack of reliable diagnostic tests for detecting vaccine serotype pneumococcal pneumonia (VTPP) remains a challenging issue in pneumococcal vaccine studies. This study assessed the performances of high-throughput nanofluidic PCR-based pneumococcal serotyping and quantification assay methods using sputum samples (the nanofluidic sputum quantitative PCR [Sp-qPCR] assay) to diagnose 13-valent pneumococcal conjugate VTPP compared with the performance of the serotype-specific urinary antigen detection (UAD) assay using urine samples. Adult pneumonia patients from Japan were enrolled in this study between September 2012 and August 2014. Sputum samples were subjected to the nanofluidic Sp-qPCR assay, quantitatively cultured, and serotyped by the Quellung reaction (SpQt). Urine samples were tested by the UAD method. The diagnostic performances of these tests were assessed using composite reference standards and Bayesian latent class models (BLCMs). Among 244 total patients, 27 (11.1%) tested positive with the UAD assay, while 16 (6.6%) and 34 (13.9%) tested positive with the SpQt and nanofluidic Sp-qPCR assays, respectively, with a cutoff value of 104 DNA copies/ml, which showed the maximum value of the Youden index. Using BLCMs, the estimated prevalence for VTPP was 12.9%, and the nanofluidic Sp-qPCR assay demonstrated the best performance (sensitivity, 90.2%; specificity, 96.9%), followed by UAD (sensitivity, 75.6%; specificity, 97.9%) and SpQt (sensitivity, 45.8%; specificity, 99.5%). However, when a higher cutoff value of 107 DNA copies/ml was applied, the performance of UAD became comparable to that of Sp-qPCR. The vaccine serotype-specific pneumococcal DNA load in sputum among UAD-positive patients was 3 logs higher than that among UAD-negative patients (P 0.036). The nanofluidic Sp-qPCR assay may be accurate and useful for detecting VTPP among adults. | |||||
書誌情報 |
Journal of Clinical Microbiology 巻 56, 号 5, p. e01874-17, 発行日 2018-05 |
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出版者 | ||||||
出版者 | American Society for Microbiology | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 00951137 | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1098660X | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1128/JCM.01874-17 | |||||
権利 | ||||||
権利情報 | c 2018 Kakiuchi et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license. |
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著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Journal of Clinical Microbiology, 56(5), e01874-17; 2018 |