WEKO3
アイテム
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Basal Autophagy Deficiency Causes Thyroid Follicular Epithelial Cell Death in Mice
http://hdl.handle.net/10069/39462
http://hdl.handle.net/10069/394628832133e-9fd2-4660-84d7-46a4ca90fd34
名前 / ファイル | ライセンス | アクション |
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Endo160_2085.pdf (636.9 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2020-07-17 | |||||
タイトル | ||||||
タイトル | Basal Autophagy Deficiency Causes Thyroid Follicular Epithelial Cell Death in Mice | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | thyrocyte | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | autophagy | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | atg5 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | knockout | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | apoptosis | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Kurashige, Tomomi
× Kurashige, Tomomi× Nakajima, Yasuyo× Shimamura, Mika× Matsuyama, Mutsumi× Yamada, Masanobu× Nakashima, Masahiro× Nagayama, Yuji |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Autophagy is a catabolic process that involves the degradation of cellular components through the lysosomal machinery, relocating nutrients from unnecessary processes to more pivotal processes required for survival. It has been reported that systemic disruption of the Atg5 or Atg7 gene, a component of autophagy, is lethal and that its tissue-specific disruption causes tissue degeneration in several organs. However, the functional significance of autophagy in the thyroid glands remains unknown. Our preliminary data imply the possible involvement of dysfunctional autophagy in radiation-induced thyroid carcinogenesis. Therefore, we evaluated the effect of Atg5 gene knockout (KO) on thyroid morphology and function. To this end, Atg5flox/flox mice were crossed with TPO-Cre mice, yielding the thyroid follicular epithelial cell (thyrocyte)?specific ATG5-deficient mice (Atg5thyr-KO/KO). Atg5 gene KO was confirmed by a lack of ATG5 expression, and disruption of autophagy was demonstrated by a decrease in microtubule-associated protein 1 light chain 3-II puncta and an increase in p62. Atg5thyr-KO/KO mice were born normally, and thyroid morphology, thyroid weights, and serum T4 and TSH levels were almost normal at 4 months. However, at 8 and 12 months, a decrease in the number of thyrocytes and an increase in TUNEL+-thyrocytes were observed in Atg5thyr-KO/KO mice even though thyroid function was still normal. The number of irregularly shaped (gourd-shaped) follicles was also increased. Excess oxidative stress was indicated by increased 8-hydroxy-2'-deoxyguanosine and 53BP1 foci in Atg5thyr-KO/KO mice. These data demonstrate that thyrocytes gradually undergo degradation/cell death in the absence of basal levels of autophagy, indicating that autophagy is critical for the quality control of thyrocytes. | |||||
書誌情報 |
Endocrinology 巻 160, 号 9, p. 2085-2092, 発行日 2019-07-17 |
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出版者 | ||||||
出版者 | Oxford University Press | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 19457170 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1210/en.2019-00312 | |||||
権利 | ||||||
権利情報 | c 2019 Endocrine Society. This is a pre-copyedited, author-produced version of an article accepted for publication in Endocrinology following peer review. The version of record Endocrinology, 160(9), pp.2085-2092; 2019 is available online at: https://doi.org/10.1210/en.2019-00312. | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Endocrinology, 160(9), pp.2085-2092; 2019 |