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Copper excess promotes propagation and induces proteomic change in root cultures of Hyoscyamus albus L.
http://hdl.handle.net/10069/36243
http://hdl.handle.net/10069/36243fda06eef-ea75-4110-b65e-bdb5c9c63210
名前 / ファイル | ライセンス | アクション |
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PPB103_1.pdf (3.3 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2018-07-01 | |||||
タイトル | ||||||
タイトル | Copper excess promotes propagation and induces proteomic change in root cultures of Hyoscyamus albus L. | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | copper | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | heavy metal | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Hyoscyamus albus | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | proteomics | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | root culture | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | root respiration | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Sako, Ari
× Sako, Ari× Kandakar, Jebunnahar× Tamari, Noriko× Higa, Ataru× Yamaguchi, Kenichi× Kitamura, Yoshie |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Hyoscyamus albus L. seedlings respond positively to copper (Cu) excess. In the present study, to understand how roots cope with Cu excess, propagation and proteome composition in the presence of Cu were examined using a root culture system. When H. albus roots were cultured in a medium without Cu, root growth deteriorated. However, in the presence of Cu, root growth increased in a concentration-dependent manner, and vigorous lateral root development was observed at 200 μM Cu. Cu accumulation in the roots increased with the Cu supply. Subcellular fractionation revealed that the highest amount of Cu was present in the cell wall-containing fraction, followed by the soluble fraction. However, the highest specific incorporation of Cu, in terms of fresh weight, was in the mitochondria-rich fraction. High Cu levels enhanced respiration activity. Comparative proteomic analysis revealed that proteins involved in carbohydrate metabolism, de novo protein synthesis, cell division, and ATP synthesis increased in abundance, whereas the proteasome decreased. These results indicate that Cu promotes propagation of H. albus roots through the activation of the energy supply and anabolism. Newly propagated root tissues and newly generated proteins that bind to Cu may provide space and reservoirs for deposition of additional Cu. | |||||
書誌情報 |
Plant Physiology and Biochemistry 巻 103, p. 1-9, 発行日 2016-06 |
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出版者 | ||||||
出版者 | Elsevier Masson SAS | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 09819428 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1016/j.plaphy.2016.02.032 | |||||
権利 | ||||||
権利情報 | (c) 2016 Elsevier Masson SAS. | |||||
権利 | ||||||
権利情報 | This manuscript version is made available under the CC-BY-NC-ND 4.0 license. | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Plant Physiology and Biochemistry, 103, pp.1-9; 2016 |