WEKO3
アイテム
{"_buckets": {"deposit": "2114f06e-b881-4150-931e-9b08914f69e5"}, "_deposit": {"created_by": 2, "id": "3511", "owners": [2], "pid": {"revision_id": 0, "type": "depid", "value": "3511"}, "status": "published"}, "_oai": {"id": "oai:nagasaki-u.repo.nii.ac.jp:00003511", "sets": ["51"]}, "author_link": ["14937", "14935", "14936"], "item_2_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2015-12-29", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "4", "bibliographicPageEnd": "215", "bibliographicPageStart": "213", "bibliographicVolumeNumber": "50", "bibliographic_titles": [{"bibliographic_title": "Fish Pathology"}]}]}, "item_2_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "A multiplex PCR assay for differentiation of Streptococcus parauberis serotypes was developed. The three primer pairs for subserotypes Ia and Ib/Ic and serotype II were designed from the serotype-specific sequences of the wzy gene in the loci for capsular polysaccharide biosynthesis. All of 188 S. parauberis isolates from Japanese flounder showed positive reaction with the expected size of PCR product for each serotype, which was consistent with the results of agglutination test using rabbit antisera. The nontypeable isolates, which are not differentiated by agglutination test, could be identified as subzero type Ib/Ic or serotype II. Other streptococci including S. parauberis derived from cow and major bacterial fish pathogens showed negative reaction.", "subitem_description_type": "Abstract"}]}, "item_2_description_63": {"attribute_name": "引用", "attribute_value_mlt": [{"subitem_description": "Fish Pathology, 50(4), pp.213-215; 2015", "subitem_description_type": "Other"}]}, "item_2_publisher_33": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "日本魚病学会"}]}, "item_2_relation_12": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type": "isIdenticalTo", "subitem_relation_type_id": {"subitem_relation_type_id_text": "10.3147/jsfp.50.213", "subitem_relation_type_select": "DOI"}}]}, "item_2_rights_13": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "c 2015 The Japanese Society of Fish Pathology"}]}, "item_2_source_id_7": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0388788X", "subitem_source_identifier_type": "ISSN"}]}, "item_2_source_id_8": {"attribute_name": "EISSN", "attribute_value_mlt": [{"subitem_source_identifier": "18817335", "subitem_source_identifier_type": "ISSN"}]}, "item_2_text_62": {"attribute_name": "出版者別言語", "attribute_value_mlt": [{"subitem_text_value": "Japanese Society of Fish Pathology"}]}, "item_2_version_type_16": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_970fb48d4fbd8a85", "subitem_version_type": "VoR"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Tu, Chuandeng"}], "nameIdentifiers": [{"nameIdentifier": "14935", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Suga, Koushirou"}], "nameIdentifiers": [{"nameIdentifier": "14936", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kanai, Kinya"}], "nameIdentifiers": [{"nameIdentifier": "14937", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2020-12-21"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "JSFP50_213.pdf", "filesize": [{"value": "493.9 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 493900.0, "url": {"label": "JSFP50_213.pdf", "url": "https://nagasaki-u.repo.nii.ac.jp/record/3511/files/JSFP50_213.pdf"}, "version_id": "f9e07c0e-505d-4be3-805e-8df3d4101165"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "Streptococcus parauberis", "subitem_subject_scheme": "Other"}, {"subitem_subject": "multiplex PCR", "subitem_subject_scheme": "Other"}, {"subitem_subject": "serotyping", "subitem_subject_scheme": "Other"}, {"subitem_subject": "streptococcosis", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "A Multiplex PCR Assay for Different-iation of Streptococcus parauberis Serotypes", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "A Multiplex PCR Assay for Different-iation of Streptococcus parauberis Serotypes"}]}, "item_type_id": "2", "owner": "2", "path": ["51"], "permalink_uri": "http://hdl.handle.net/10069/36676", "pubdate": {"attribute_name": "公開日", "attribute_value": "2016-08-27"}, "publish_date": "2016-08-27", "publish_status": "0", "recid": "3511", "relation": {}, "relation_version_is_last": true, "title": ["A Multiplex PCR Assay for Different-iation of Streptococcus parauberis Serotypes"], "weko_shared_id": -1}
A Multiplex PCR Assay for Different-iation of Streptococcus parauberis Serotypes
http://hdl.handle.net/10069/36676
http://hdl.handle.net/10069/36676b680c5e0-0d6e-46c0-a0bb-f66a38471ae0
名前 / ファイル | ライセンス | アクション |
---|---|---|
JSFP50_213.pdf (493.9 kB)
|
|
Item type | 学術雑誌論文 / Journal Article(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2016-08-27 | |||||
タイトル | ||||||
タイトル | A Multiplex PCR Assay for Different-iation of Streptococcus parauberis Serotypes | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Streptococcus parauberis | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | multiplex PCR | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | serotyping | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | streptococcosis | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Tu, Chuandeng
× Tu, Chuandeng× Suga, Koushirou× Kanai, Kinya |
|||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | A multiplex PCR assay for differentiation of Streptococcus parauberis serotypes was developed. The three primer pairs for subserotypes Ia and Ib/Ic and serotype II were designed from the serotype-specific sequences of the wzy gene in the loci for capsular polysaccharide biosynthesis. All of 188 S. parauberis isolates from Japanese flounder showed positive reaction with the expected size of PCR product for each serotype, which was consistent with the results of agglutination test using rabbit antisera. The nontypeable isolates, which are not differentiated by agglutination test, could be identified as subzero type Ib/Ic or serotype II. Other streptococci including S. parauberis derived from cow and major bacterial fish pathogens showed negative reaction. | |||||
書誌情報 |
Fish Pathology 巻 50, 号 4, p. 213-215, 発行日 2015-12-29 |
|||||
出版者 | ||||||
出版者 | 日本魚病学会 | |||||
出版者別言語 | ||||||
Japanese Society of Fish Pathology | ||||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0388788X | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 18817335 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.3147/jsfp.50.213 | |||||
権利 | ||||||
権利情報 | c 2015 The Japanese Society of Fish Pathology | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Fish Pathology, 50(4), pp.213-215; 2015 |