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Functional maintenance of calcium store by ShcB adaptor protein in cerebellar Purkinje cells
http://hdl.handle.net/10069/40264
http://hdl.handle.net/10069/40264458bf7ab-6dde-427f-874e-10180b34a1c4
名前 / ファイル | ライセンス | アクション |
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SciRep10_14475.pdf.pdf (8.4 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2020-09-18 | |||||
タイトル | ||||||
タイトル | Functional maintenance of calcium store by ShcB adaptor protein in cerebellar Purkinje cells | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Kakizawa, Sho
× Kakizawa, Sho× Kishimoto, Yasushi× Yamamoto, Shinichiro× Onga, Kazuko× Yasuda, Kunihiko× Miyamoto, Yoshiaki× Watanabe, Masahiko× Sakai, Ryuichi× Mori, Nozomu |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Intracellular Ca2+ levels are changed by influx from extracellular medium and release from intracellular stores. In the central nervous systems, Ca2+ release is involved in various physiological events, such as neuronal excitability and transmitter release. Although stable Ca2+ release in response to stimulus is critical for proper functions of the nervous systems, regulatory mechanisms relating to Ca2+ release are not fully understood in central neurons. Here, we demonstrate that ShcB, an adaptor protein expressed in central neurons, has an essential role in functional maintenance of Ca2+ store in cerebellar Purkinje cells (PCs). ShcB-knockout (KO)mice showed defects in cerebellar-dependent motor function and long-term depression (LTD) at cerebellar synapse. The reduced LTD was accompanied with an impairment of intracellular Ca2+ release. Although the expression of Ca2+ release channels and morphology of Ca2+ store looked intact, content of intracellular Ca2+ store and activity of sarco/endoplasmic reticular Ca2+-ATPase (SERCA) were largely decreased in the ShcB-deficient cerebellum. Furthermore, when ShcB was ectopically expressed in the ShcB-KO PCs, the Ca2+ release and its SERCA-dependent component were restored. These data indicate that ShcB plays a key role in the functional maintenance of ER Ca2+ store in central neurons through regulation of SERCA activity. | |||||
書誌情報 |
Scientific Reports 巻 10, 号 1, p. 14475, 発行日 2020-09-02 |
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出版者 | ||||||
出版者 | Springer Nature | |||||
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収録物識別子タイプ | ISSN | |||||
収録物識別子 | 20452322 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1038/s41598-020-71414-y | |||||
権利 | ||||||
権利情報 | c The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Scientific Reports, 10(1), art.no.14475; 2020 |