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Recently, a protocol for self-organizing optic cup morphogenesis in threedimensional culture was reported, and it was suggested that ocular tissue derived from neural ectoderm could be differentiated. We demonstrated that a CE-like double-layered structure could be induced in simple culture by using a modified Eiraku differentiation protocol. RESULTS. Differentiation of a CE-like double-layered structure could be promoted by glycogen synthase kinase 3β (GSK-3β) inhibitor. Connexin43 and aquaporin1 were expressed in both thin layers, and induced CE-like cells expressed ciliary marker genes, such as cyclinD2, zic1, tgfb2, aldh1a3, wfdc1, otx1, BMP4, and BMP7. Increases in cytoplasmic and nuclear β-catenin in aggregates of the CE-like double-layered structure were confirmed by Western blot analysis. In addition, tankyrase inhibitor prevented the induction of the CE-like double-layered structure by GSK-3β inhibitor. 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Induction of Functional 3D Ciliary Epithelium?Like Structure From Mouse Induced Pluripotent Stem Cells
http://hdl.handle.net/10069/36918
http://hdl.handle.net/10069/3691820ffc48f-d192-43a8-bf2f-28e5c443c444
名前 / ファイル | ライセンス | アクション |
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IOVS57_153.pdf (1.4 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2016-10-31 | |||||
タイトル | ||||||
タイトル | Induction of Functional 3D Ciliary Epithelium?Like Structure From Mouse Induced Pluripotent Stem Cells | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | ciliary epithelium | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | iPS | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | GSK-3β | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | β-catenin | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Kinoshita, Hirofumi
× Kinoshita, Hirofumi× Suzuma, Kiyoshi× Kaneko, Jun× Mandai, Michiko× Kitaoka, Takashi× Takahashi, Masayo |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | PURPOSE. To generate ciliary epithelium (CE) from mouse induced pluripotent stem (iPS) cells. METHODS. Recently, a protocol for self-organizing optic cup morphogenesis in threedimensional culture was reported, and it was suggested that ocular tissue derived from neural ectoderm could be differentiated. We demonstrated that a CE-like double-layered structure could be induced in simple culture by using a modified Eiraku differentiation protocol. RESULTS. Differentiation of a CE-like double-layered structure could be promoted by glycogen synthase kinase 3β (GSK-3β) inhibitor. Connexin43 and aquaporin1 were expressed in both thin layers, and induced CE-like cells expressed ciliary marker genes, such as cyclinD2, zic1, tgfb2, aldh1a3, wfdc1, otx1, BMP4, and BMP7. Increases in cytoplasmic and nuclear β-catenin in aggregates of the CE-like double-layered structure were confirmed by Western blot analysis. In addition, tankyrase inhibitor prevented the induction of the CE-like double-layered structure by GSK-3β inhibitor. Dye movement from pigmented cells to nonpigmented cells in the mouse iPS cell?derived CE-like structure was observed in a fluid movement experiment, consistent with the physiological function of CE in vivo. CONCLUSIONS. We could differentiate CE from mouse iPS cells in the present study. In the future, we hope that this CE-like complex will become useful as a graft for transplantation therapy in pathologic ocular hypotension due to CE dysfunction, and as a screening tool for the development of drugs for diseases associated with CE function. | |||||
書誌情報 |
Investigative Opthalmology & Visual Science 巻 57, 号 1, p. 153-161, 発行日 2016-01 |
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出版者 | ||||||
出版者 | Association for Research in Vision and Ophthalmology Inc. | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 15525783 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1167/iovs.15-17610 | |||||
権利 | ||||||
権利情報 | c 2016, Association for Research in Vision and Ophthalmology Inc. All rights reserved. | |||||
権利 | ||||||
権利情報 | This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Investigative Opthalmology & Visual Science, 57(1), pp.153-161; 2016 |