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Quantitative RT-PCR evaluation of a rapid influenza antigen test for efficient diagnosis of influenza virus infection
http://hdl.handle.net/10069/35305
http://hdl.handle.net/10069/35305eff5c85b-18a9-46f7-86eb-0c7406685675
名前 / ファイル | ライセンス | アクション |
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JVM212_76.pdf (91.6 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2015-05-13 | |||||
タイトル | ||||||
タイトル | Quantitative RT-PCR evaluation of a rapid influenza antigen test for efficient diagnosis of influenza virus infection | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Influenza virus | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Quantitative RT-PCR | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Rapid influenza antigen detection test | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Tsushima, Yuko
× Tsushima, Yuko× Uno, Naoki× Sasaki, Daisuke× Morinaga, Yoshitomo× Hasegawa, Hiroo× Yanagihara, Katsunori |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Influenza virus infection is diagnosed in most cases using a rapid influenza antigen diagnostic test (RIDT). However, false-negative results are a major concern. By contrast, the nucleic acid amplification test offers high sensitivity and therefore can aid the interpretation of negative RIDT results. In this study, influenza viral loads were quantified with quantitative reverse transcription-polymerase chain reaction (qRT-PCR) using viral suspensions left over after RIDT, and the performance of both methods was evaluated. qRT-PCR detected as few as 103copies/mL of influenza viruses A and B, whereas RIDT showed negative results for viral loads less than 107 and 105copies/mL of influenza viruses A and B, respectively. These results indicate that small quantities of the virus that cause false-negative RIDT results can be detected efficiently with qRT-PCR follow-up. In addition, influenza A virus subtype was determined using qRT-PCR. | |||||
書誌情報 |
Journal of Virological Methods 巻 212, p. 76-79, 発行日 2015-02 |
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出版者 | ||||||
出版者 | Elsevier | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 01660934 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1016/j.jviromet.2014.10.019 | |||||
権利 | ||||||
権利情報 | c 2014 Elsevier B.V. | |||||
権利 | ||||||
権利情報 | NOTICE: this is the author’s version of a work that was accepted for publication in Journal of Virological Methods. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Journal of Virological Methods, 212, (2015) | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Journal of Virological Methods, 212, pp.76-79; 2015 |