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Development of monoclonal antibodies against Plasmodium falciparum thioredoxin peroxidase 1 and its possible application for malaria diagnosis
http://hdl.handle.net/10069/35455
http://hdl.handle.net/10069/354552eaa9798-8d2d-4a1e-9f4c-ae0217105de7
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Supple.pdf (207.8 kB)
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ExPara154_62.pdf (378.5 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2016-08-01 | |||||
タイトル | ||||||
タイトル | Development of monoclonal antibodies against Plasmodium falciparum thioredoxin peroxidase 1 and its possible application for malaria diagnosis | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Malaria diagnosis | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Plasmodium falciparum | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Rapid diagnostic test | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Thioredoxin peroxidase 1 | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Hakimi, Hassan
× Hakimi, Hassan× Goto, Yasuyuki× Suganuma, Keisuke× Angeles, Jose Ma M.× Kawai, Satoru× Inoue, Noboru× Kawazu, Shin-ichiro |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Rapid diagnostic tests (RDTs) have been considered as an ideal alternative for light microscopy to detect malaria parasites especially in remote areas. The development and improvement of RDTs is an area of intensive research in the last decade. To date, few parasite proteins have been targeted in RDTs which are known to have certain deficiencies and made the researchers to look for other promising candidates to address this problem. Plasmodium falciparum thioredoxin peroxidase 1 (PfTPx-1) is abundantly expressed in the cytoplasm of the parasite and well conserved across Plasmodium species, making this antigen a promising target for malaria diagnosis. Several monoclonal antibodies (mAbs) were produced against PfTPx-1. The binding affinities of mAbs were measured. Several immunochromatographic tests (ICTs) were developed using different combination of mAbs. All mAbs showed promising affinities to be used for diagnosis. The sensitivities of ICTs were evaluated using recombinant PfTPx-1 whose results lead us to the preparation of 4 different ICTs. These tests showed positive reaction with P. falciparum in vitro culture supernatant indicating the release of PfTPx-1 during schizont rupture. Altogether, these findings suggest that PfTPx-1 is a promising biomarker to diagnose P. falciparum infection. However, the diagnostic performance of this antigen should be further validated using clinical samples. | |||||
書誌情報 |
Experimental Parasitology 巻 154, p. 62-66, 発行日 2015-07 |
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出版者 | ||||||
出版者 | Academic Press Inc. | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 00144894 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1016/j.exppara.2015.04.018 | |||||
権利 | ||||||
権利情報 | c 2015 Elsevier Inc. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Experimental Parasitology, 154, pp.62-66; 2015 |