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Effect of age on the sensitivity of the rat thyroid gland to ionizing radiation
http://hdl.handle.net/10069/35499
http://hdl.handle.net/10069/354995da928c7-ce4f-48b4-95c2-64dc9ff3cafc
名前 / ファイル | ライセンス | アクション |
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JRR56_493.pdf (703.6 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2015-06-29 | |||||
タイトル | ||||||
タイトル | Effect of age on the sensitivity of the rat thyroid gland to ionizing radiation | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Apoptosis | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Autophagy | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | p53 binding protein 1 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Rat | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Thyroid | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | X-ray | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Matsuu-Matsuyama, Mutsumi
× Matsuu-Matsuyama, Mutsumi× Shichijo, Kazuko× Okaichi, Kumio× Kurashige, Tomomi× Kondo, Hisayoshi× Miura, Shiro× Nakashima, Masahiro |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Exposure to ionizing radiation during childhood is a well-known risk factor for thyroid cancer. Our study evaluated the effect of age on the radiosensitivity of rat thyroid glands. Four-week-old (4W), 7 -week-old (7W), and 8-month-old (8M) male Wistar rats were exposed to 8 Gy of whole-body X-ray irradiation. Thyroids were removed 3-72 h after irradiation, and non-irradiated thyroids served as controls. Ki67-positivity and p53 binding protein 1 (53BP1) focus formation (a DNA damage response) were evaluated via immunohistochemistry. Amounts of proteins involved in DNA damage response (p53, p53 phosphorylated at serine 15, p21), apoptosis (cleaved caspase-3), and autophagy (LC3, p62) were determined via western blotting. mRNA levels of 84 key autophagy-related genes were quantified using polymerase chain reaction arrays. Ki67-positive cells in 4W (with high proliferative activity) and 7W thyroids significantly decreased in number post-irradiation. The number of 53BP1 foci and amount of p53 phosphorylated at serine 15 increased 3 h after irradiation, regardless of age. No increase in apoptosis or in the levels of p53, p21 or cleaved caspase-3 was detected for any ages. Levels of LC3-II and p62 increased in irradiated 4W but not 8M thyroids, whereas expression of several autophagy-related genes was higher in 4W than 8M irradiated thyroids. Irradiation increased the expression of genes encoding pro-apoptotic proteins in both 4W and 8M thyroids. In summary, no apoptosis or p53 accumulation was noted, despite the expression of some pro-apoptotic genes in immature and adult thyroids. Irradiation induced autophagy in immature, but not in adult, rat thyroids. | |||||
書誌情報 |
Journal of Radiation Research 巻 56, 号 3, p. 493-501, 発行日 2015-05 |
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出版者 | ||||||
出版者 | 日本放射線影響学会 | |||||
出版者別言語 | ||||||
Japan Radiation Research Society | ||||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 04493060 | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 13499157 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1093/jrr/rrv003 | |||||
権利 | ||||||
権利情報 | c The Author 2015. Published by Oxford University Press on behalf of The Japan Radiation Research Society and Japanese Society for Radiation Oncology. | |||||
権利 | ||||||
権利情報 | This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Journal of Radiation Research, 56(3), pp.493-501; 2015 |