WEKO3
アイテム
{"_buckets": {"deposit": "61056c19-4337-4205-b8fc-06fa2e28b536"}, "_deposit": {"created_by": 2, "id": "4373", "owners": [2], "pid": {"revision_id": 0, "type": "depid", "value": "4373"}, "status": "published"}, "_oai": {"id": "oai:nagasaki-u.repo.nii.ac.jp:00004373", "sets": ["74"]}, "author_link": ["17526", "17527", "17524", "17528", "17529", "17525"], "item_2_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2015-06-30", "bibliographicIssueDateType": "Issued"}, "bibliographicPageEnd": "147", "bibliographicPageStart": "139", "bibliographicVolumeNumber": "881", "bibliographic_titles": [{"bibliographic_title": "Analytica Chimica Acta"}]}]}, "item_2_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "A fast, simple, and sensitive flow injection analysis method was developed for the measurement of semicarbazide-sensitive amine oxidase (SSAO) activity in human serum. Benzaldehyde, generated by the action of SSAO after incubation of serum with benzylamine, was derivatized with a novel aromatic aldehyde-specific reagent (1,2-diaminoanthraquinone) and the fluorescent product was measured by fluorescence detection at excitation and emission wavelengths of 390 and 570nm, respectively. Serum SSAO activity was defined as benzaldehyde (nmol) formed per milliliter serum per hour. The method was linear over SSAO activity of 0.2-150.0nmolmL-1h-1 with a detection limit of 0.06nmolmL-1h-1. The %RSD of intra-day and inter-day precision did not exceed 9.4% and the accuracy ranged from -6.5 to -0.6%. The method was applied for the determination of the serum SSAO activity in healthy controls (C, n=24) and diabetes mellitus patients (DM, n=18). It was demonstrated that the activity (mean±SE) of SSAO in diabetics sera was significantly higher than that in healthy subjects\u0027 ones (DM; 73.3±1.8nmolmL-1h-1 vs C; 58.9±2.2nmolmL-1h-1, P\u003c0.01).", "subitem_description_type": "Abstract"}]}, "item_2_description_63": {"attribute_name": "引用", "attribute_value_mlt": [{"subitem_description": "Analytica Chimica Acta, 881, pp.139-147; 2015", "subitem_description_type": "Other"}]}, "item_2_publisher_33": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "Elsevier B.V."}]}, "item_2_relation_12": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type": "isVersionOf", "subitem_relation_type_id": {"subitem_relation_type_id_text": "10.1016/j.aca.2015.04.006", "subitem_relation_type_select": "DOI"}}]}, "item_2_rights_13": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "c 2015 Elsevier B.V. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/"}]}, "item_2_source_id_7": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "00032670", "subitem_source_identifier_type": "ISSN"}]}, "item_2_version_type_16": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_ab4af688f83e57aa", "subitem_version_type": "AM"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "El-Maghrabey, Mahmoud H."}], "nameIdentifiers": [{"nameIdentifier": "17524", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kishikawa, Naoya"}], "nameIdentifiers": [{"nameIdentifier": "17525", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Ohyama, Kaname"}], "nameIdentifiers": [{"nameIdentifier": "17526", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Imazato, Takahiro"}], "nameIdentifiers": [{"nameIdentifier": "17527", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Ueki, Yukitaka"}], "nameIdentifiers": [{"nameIdentifier": "17528", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kuroda, Naotaka"}], "nameIdentifiers": [{"nameIdentifier": "17529", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2020-12-21"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "ACA881_139.pdf", "filesize": [{"value": "471.3 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 471300.0, "url": {"label": "ACA881_139.pdf", "url": "https://nagasaki-u.repo.nii.ac.jp/record/4373/files/ACA881_139.pdf"}, "version_id": "fc100191-bb4c-4308-89b6-1a789e8453b0"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "1,2-Diaminoanthraquinone", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Benzaldehyde", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Flow injection analysis", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Fluorescence detection", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Semicarbazide-sensitive amine oxidase activity", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Determination of human serum semicarbazide-sensitive amine oxidase activity via flow injection analysis with fluorescence detection after online derivatization of the enzymatically produced benzaldehyde with 1,2-diaminoanthraquinone", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Determination of human serum semicarbazide-sensitive amine oxidase activity via flow injection analysis with fluorescence detection after online derivatization of the enzymatically produced benzaldehyde with 1,2-diaminoanthraquinone"}]}, "item_type_id": "2", "owner": "2", "path": ["74"], "permalink_uri": "http://hdl.handle.net/10069/35501", "pubdate": {"attribute_name": "公開日", "attribute_value": "2017-07-01"}, "publish_date": "2017-07-01", "publish_status": "0", "recid": "4373", "relation": {}, "relation_version_is_last": true, "title": ["Determination of human serum semicarbazide-sensitive amine oxidase activity via flow injection analysis with fluorescence detection after online derivatization of the enzymatically produced benzaldehyde with 1,2-diaminoanthraquinone"], "weko_shared_id": -1}
Determination of human serum semicarbazide-sensitive amine oxidase activity via flow injection analysis with fluorescence detection after online derivatization of the enzymatically produced benzaldehyde with 1,2-diaminoanthraquinone
http://hdl.handle.net/10069/35501
http://hdl.handle.net/10069/3550194c0d666-fd0c-43d0-8d87-452d0cb95d71
名前 / ファイル | ライセンス | アクション |
---|---|---|
ACA881_139.pdf (471.3 kB)
|
|
Item type | 学術雑誌論文 / Journal Article(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2017-07-01 | |||||
タイトル | ||||||
タイトル | Determination of human serum semicarbazide-sensitive amine oxidase activity via flow injection analysis with fluorescence detection after online derivatization of the enzymatically produced benzaldehyde with 1,2-diaminoanthraquinone | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 1,2-Diaminoanthraquinone | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Benzaldehyde | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Flow injection analysis | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Fluorescence detection | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Semicarbazide-sensitive amine oxidase activity | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
El-Maghrabey, Mahmoud H.
× El-Maghrabey, Mahmoud H.× Kishikawa, Naoya× Ohyama, Kaname× Imazato, Takahiro× Ueki, Yukitaka× Kuroda, Naotaka |
|||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | A fast, simple, and sensitive flow injection analysis method was developed for the measurement of semicarbazide-sensitive amine oxidase (SSAO) activity in human serum. Benzaldehyde, generated by the action of SSAO after incubation of serum with benzylamine, was derivatized with a novel aromatic aldehyde-specific reagent (1,2-diaminoanthraquinone) and the fluorescent product was measured by fluorescence detection at excitation and emission wavelengths of 390 and 570nm, respectively. Serum SSAO activity was defined as benzaldehyde (nmol) formed per milliliter serum per hour. The method was linear over SSAO activity of 0.2-150.0nmolmL-1h-1 with a detection limit of 0.06nmolmL-1h-1. The %RSD of intra-day and inter-day precision did not exceed 9.4% and the accuracy ranged from -6.5 to -0.6%. The method was applied for the determination of the serum SSAO activity in healthy controls (C, n=24) and diabetes mellitus patients (DM, n=18). It was demonstrated that the activity (mean±SE) of SSAO in diabetics sera was significantly higher than that in healthy subjects' ones (DM; 73.3±1.8nmolmL-1h-1 vs C; 58.9±2.2nmolmL-1h-1, P<0.01). | |||||
書誌情報 |
Analytica Chimica Acta 巻 881, p. 139-147, 発行日 2015-06-30 |
|||||
出版者 | ||||||
出版者 | Elsevier B.V. | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 00032670 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1016/j.aca.2015.04.006 | |||||
権利 | ||||||
権利情報 | c 2015 Elsevier B.V. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Analytica Chimica Acta, 881, pp.139-147; 2015 |