WEKO3
アイテム
{"_buckets": {"deposit": "d67d7bff-a9af-4493-8bc7-0afdb4e30c71"}, "_deposit": {"created_by": 2, "id": "4581", "owners": [2], "pid": {"revision_id": 0, "type": "depid", "value": "4581"}, "status": "published"}, "_oai": {"id": "oai:nagasaki-u.repo.nii.ac.jp:00004581", "sets": ["51"]}, "author_link": ["18180", "18178", "18181", "18179", "18182"], "item_2_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2008-09", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "9", "bibliographicPageEnd": "982", "bibliographicPageStart": "976", "bibliographicVolumeNumber": "86", "bibliographic_titles": [{"bibliographic_title": "Canadian Journal of Zoology"}]}]}, "item_2_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "The populations of the only known self-fertilizing vertebrate Kryptolebias marmoratus (Poey, 1880) (formerly known as Rivulus marmoratus Poey, 1880; Cyprinodontiformes: Aplocheilidae) usually consist of different homozygous lineages; however, heterozygous individuals are found occasionally and ratios of homozygosity and heterozygosity in populations are dependent on the proportion of males. However, it is still unclear what impact male-mediated genetic diversity has on the phenotype of K. marmoratus. To clarify this, we attempted outcrossing between male and hermaphrodite of two different clonal strains with different life-history traits using artificial insemination, and examined the genotypes and growth of the hybrid F2 generation. We detected genetic differences between the two clonal strains using amplified fragment length polymorphism (AFLP) analysis with 3 primer combinations, and then obtained 11 AFLP markers. From a total of 31 artificial inseminations with two clonal strains, 1 of 13 hatched fish clearly indicated heterozygosity. The hybrid F2 generations were also heterozygous. Moreover, the growths of the hybrid F2 generation were intermediate of the parental strains from days 0 to 30. Therefore, outcrossing changes genetic architecture and the new genotypes potentially result in new phenotypes of the subsequent generations of K. marmoratus. It may also play a role in adaptation to new environments and the facilitation of local adaptation.", "subitem_description_type": "Abstract"}]}, "item_2_description_63": {"attribute_name": "引用", "attribute_value_mlt": [{"subitem_description": "Canadian Journal of Zoology, 86(9), pp.976-982; 2008", "subitem_description_type": "Other"}]}, "item_2_publisher_33": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "National Research Council of Canada"}]}, "item_2_relation_12": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type": "isVersionOf", "subitem_relation_type_id": {"subitem_relation_type_id_text": "10.1139/Z08-075", "subitem_relation_type_select": "DOI"}}]}, "item_2_rights_13": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "c 2008 NRC Research Press"}]}, "item_2_source_id_7": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "00084301", "subitem_source_identifier_type": "ISSN"}]}, "item_2_source_id_8": {"attribute_name": "EISSN", "attribute_value_mlt": [{"subitem_source_identifier": "14803283", "subitem_source_identifier_type": "ISSN"}]}, "item_2_version_type_16": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_ab4af688f83e57aa", "subitem_version_type": "AM"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Nakamura, Yukino"}], "nameIdentifiers": [{"nameIdentifier": "18178", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Suga, Koushirou"}], "nameIdentifiers": [{"nameIdentifier": "18179", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Sakakura, Yoshitaka"}], "nameIdentifiers": [{"nameIdentifier": "18180", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Sakamoto, Takashi"}], "nameIdentifiers": [{"nameIdentifier": "18181", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Hagiwara, Atsushi"}], "nameIdentifiers": [{"nameIdentifier": "18182", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2020-12-21"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "CJZ86_976.pdf", "filesize": [{"value": "286.7 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 286700.0, "url": {"label": "CJZ86_976.pdf", "url": "https://nagasaki-u.repo.nii.ac.jp/record/4581/files/CJZ86_976.pdf"}, "version_id": "2cf6280c-4025-46b6-b24f-a7f7d842f502"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Genetic and growth differences in the outcrossings between two clonal strains of the self-fertilizing mangrove killifish", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Genetic and growth differences in the outcrossings between two clonal strains of the self-fertilizing mangrove killifish"}]}, "item_type_id": "2", "owner": "2", "path": ["51"], "permalink_uri": "http://hdl.handle.net/10069/35714", "pubdate": {"attribute_name": "公開日", "attribute_value": "2015-07-31"}, "publish_date": "2015-07-31", "publish_status": "0", "recid": "4581", "relation": {}, "relation_version_is_last": true, "title": ["Genetic and growth differences in the outcrossings between two clonal strains of the self-fertilizing mangrove killifish"], "weko_shared_id": -1}
Genetic and growth differences in the outcrossings between two clonal strains of the self-fertilizing mangrove killifish
http://hdl.handle.net/10069/35714
http://hdl.handle.net/10069/357141ca0bdef-0be6-44e0-aa57-a142dd256eda
名前 / ファイル | ライセンス | アクション |
---|---|---|
CJZ86_976.pdf (286.7 kB)
|
|
Item type | 学術雑誌論文 / Journal Article(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2015-07-31 | |||||
タイトル | ||||||
タイトル | Genetic and growth differences in the outcrossings between two clonal strains of the self-fertilizing mangrove killifish | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Nakamura, Yukino
× Nakamura, Yukino× Suga, Koushirou× Sakakura, Yoshitaka× Sakamoto, Takashi× Hagiwara, Atsushi |
|||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | The populations of the only known self-fertilizing vertebrate Kryptolebias marmoratus (Poey, 1880) (formerly known as Rivulus marmoratus Poey, 1880; Cyprinodontiformes: Aplocheilidae) usually consist of different homozygous lineages; however, heterozygous individuals are found occasionally and ratios of homozygosity and heterozygosity in populations are dependent on the proportion of males. However, it is still unclear what impact male-mediated genetic diversity has on the phenotype of K. marmoratus. To clarify this, we attempted outcrossing between male and hermaphrodite of two different clonal strains with different life-history traits using artificial insemination, and examined the genotypes and growth of the hybrid F2 generation. We detected genetic differences between the two clonal strains using amplified fragment length polymorphism (AFLP) analysis with 3 primer combinations, and then obtained 11 AFLP markers. From a total of 31 artificial inseminations with two clonal strains, 1 of 13 hatched fish clearly indicated heterozygosity. The hybrid F2 generations were also heterozygous. Moreover, the growths of the hybrid F2 generation were intermediate of the parental strains from days 0 to 30. Therefore, outcrossing changes genetic architecture and the new genotypes potentially result in new phenotypes of the subsequent generations of K. marmoratus. It may also play a role in adaptation to new environments and the facilitation of local adaptation. | |||||
書誌情報 |
Canadian Journal of Zoology 巻 86, 号 9, p. 976-982, 発行日 2008-09 |
|||||
出版者 | ||||||
出版者 | National Research Council of Canada | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 00084301 | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 14803283 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1139/Z08-075 | |||||
権利 | ||||||
権利情報 | c 2008 NRC Research Press | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Canadian Journal of Zoology, 86(9), pp.976-982; 2008 |