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Effect of X-Irradiation at Different Stages in the Cell Cycle on Individual Cell?Based Kinetics in an Asynchronous Cell Population
http://hdl.handle.net/10069/35810
http://hdl.handle.net/10069/358105c46de84-16cb-441f-a2cf-cd637703a219
名前 / ファイル | ライセンス | アクション |
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PLoS10_128090.pdf (5.3 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2015-09-03 | |||||
タイトル | ||||||
タイトル | Effect of X-Irradiation at Different Stages in the Cell Cycle on Individual Cell?Based Kinetics in an Asynchronous Cell Population | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Tsuchida, Eri
× Tsuchida, Eri× Kaida, Atsushi× Pratama, Endrawan× Ikeda, Masa-Aki× Suzuki, Keiji× Harada, Kiyoshi× Miura, Masahiko |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Using an asynchronously growing cell population, we investigated how X-irradiation at different stages of the cell cycle influences individual cell-based kinetics. To visualize the cellcycle phase, we employed the fluorescent ubiquitination-based cell cycle indicator (Fucci). After 5 Gy irradiation, HeLa cells no longer entered M phase in an order determined by their previous stage of the cell cycle, primarily because green phase (S and G2) was less prolonged in cells irradiated during the red phase (G1) than in those irradiated during the green phase. Furthermore, prolongation of the green phase in cells irradiated during the red phase gradually increased as the irradiation timing approached late G1 phase. The results revealed that endoreduplication rarely occurs in this cell line under the conditions we studied. We next established a method for classifying the green phase into early S, mid S, late S, and G2 phases at the time of irradiation, and then attempted to estimate the duration of G2 arrest based on certain assumptions. The value was the largest when cells were irradiated in mid or late S phase and the smallest when they were irradiated in G1 phase. In this study, by closely following individual cells irradiated at different cell-cycle phases, we revealed for the first time the unique cell-cycle kinetics in HeLa cells that follow irradiation. | |||||
書誌情報 |
PLOS ONE 巻 10, 号 6, p. e0128090, 発行日 2015-06-18 |
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出版者 | ||||||
出版者 | Public Library of Science | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 19326203 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1371/journal.pone.0128090 | |||||
権利 | ||||||
権利情報 | c 2015 Tsuchida et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | PLOS ONE, 10(6), e0128090; 2015 |