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Factors contribute to efficiency of specimen concentration of Mycobacterium tuberculosis by centrifugation and magnetic beads
http://hdl.handle.net/10069/35813
http://hdl.handle.net/10069/35813d1206bb1-2162-4442-80fb-696748263393
名前 / ファイル | ライセンス | アクション |
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IJM4_245.pdf (722.9 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2015-09-03 | |||||
タイトル | ||||||
タイトル | Factors contribute to efficiency of specimen concentration of Mycobacterium tuberculosis by centrifugation and magnetic beads | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Centrifugation | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Concentration rate | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Magnetic beads | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Recovery rate | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | THP-1 cells | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Yoshimatsu, Shoji
× Yoshimatsu, Shoji× Kato-Matsumaru, Tomoko× Aono, Akio× Chikamatsu, Kinuyo× Yamada, Hiroyuki× Mitarai, Satoshi |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Background: A concentration of specimen is recommended for the effective recovery of Mycobacterium tuberculosis (MTB), but the bacteriological efficiency is not well evaluated. The present study evaluated the factors contributing to concentration efficiency of centrifugation and bead-based technique (TB-Beads; Microsens, UK) to recover MTB by using simple in vitro specimens. Methods: Four specimens were prepared (6.5×103; 8.1×104; 7.9×105; and 6.4×106cfu/mL) of different concentrations with or without 5×104 of THP-1 cells (RIKEN BRC, Japan). Specimens were subjected to centrifugation at 2000, 3000, and 4000g for 15min, and to TB-Beads. The concentration and recovery rate were calculated to evaluate the efficiency of each method. Results: The specimens containing a higher number of bacteria and THP-1 cells had a tendency to yield a higher concentration and recovery rate (p=0.001-0.083). MTB was recovered more efficiently with THP-1 cells from the 6.5×103cfu/mL specimen by centrifugation (p?0.001) than without them; 24.7-54.4% of MTB were recovered with THP-1 cells by centrifugation at 3000g for 15min, while the recovery using TB-Beads was a maximum of 12.7%. Conclusions: The efficiency of centrifugation depends on the bacterial density and the co-existence of THP-1 cells. The efficiency of TB-Beads was not as high as centrifugation. | |||||
書誌情報 |
International Journal of Mycobacteriology 巻 4, 号 3, p. 245-249, 発行日 2015-09 |
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出版者 | ||||||
出版者 | Elsevier Inc. | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 22125531 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1016/j.ijmyco.2015.05.014 | |||||
権利 | ||||||
権利情報 | c 2015 Asian-African Society for Mycobacteriology. Published by Elsevier Ltd. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/3.0/). | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | International Journal of Mycobacteriology, 4(3), pp.245-249; 2015 |