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Human BST-2/tetherin inhibits Junin virus release from host cells and its inhibition is partially counteracted by viral nucleoprotein
http://hdl.handle.net/10069/40274
http://hdl.handle.net/10069/40274a1bd1567-8b33-4b16-8508-40e1c20e526a
名前 / ファイル | ライセンス | アクション |
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ISYK1266_Rajabali.pdf (1.8 MB)
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Item type | 学位論文 / Thesis or Dissertation(1) | |||||
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公開日 | 2020-09-23 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Human BST-2/tetherin inhibits Junin virus release from host cells and its inhibition is partially counteracted by viral nucleoprotein | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Junin virus | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Arenaviridae | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | human BST-2 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | innate immunity | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Tetherin | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||
資源タイプ | doctoral thesis | |||||
アクセス権 | ||||||
アクセス権 | open access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
著者 |
Rajabali, Zadeh Vahid
× Rajabali, Zadeh Vahid |
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著者別名 | ||||||
姓名 | ラジャバリ ザデ, バヒッド | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Bone marrow stromal cell antigen-2 (BST-2), also known as tetherin, is an interferon-inducible membrane-associated protein. It effectively targets enveloped viruses at the release step of progeny viruses from host cells, thereby restricting the further spread of viral infection. Junin virus (JUNV) is a member of Arenaviridae, which causes Argentine haemorrhagic fever that is associated with a high rate of mortality. In this study, we examined the effect of human BST-2 on the replication and propagation of JUNV. The production of JUNV Z-mediated virus-like particles (VLPs) was significantly inhibited by over-expression of BST-2. Electron microscopy analysis revealed that BST-2 functions by forming a physical link that directly retains VLPs on the cell surface. Infection using JUNV showed that infectious JUNV production was moderately inhibited by endogenous or exogenous BST-2. We also observed that JUNV infection triggers an intense interferon response, causing an upregulation of BST-2, in infected cells. However, the expression of cell surface BST-2 was reduced upon infection. Furthermore, the expression of JUNV nucleoprotein (NP) partially recovered VLP production from BST-2 restriction, suggesting that the NP functions as an antagonist against antiviral effect of BST-2. We further showed that JUNV NP also rescued the production of Ebola virus VP40-mediated VLP from BST-2 restriction as a broad spectrum BST-2 antagonist. To our knowledge, this is the first report showing that an arenavirus protein counteracts the antiviral function of BST-2. | |||||
内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 長崎大学学位論文 学位記番号:博(医歯薬)甲第1266号 学位授与年月日:令和2年9月18日 | |||||
内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Author: Vahid Rajabali Zadeh?, Shuzo Urata?, Miako Sakaguchi, Jiro Yasuda? | |||||
内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Citation: Journal of General Virology, 101(6), pp.573-586; 2020 | |||||
書誌情報 |
Journal of General Virology 巻 101, 号 6, p. 573-586, 発行日 2020-09-18 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 00221317 | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 14652099 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1099/jgv.0.001414 | |||||
権利 | ||||||
権利情報 | c 2020 The Authors. The definitive peer reviewed, edited version of this article is published in Journal of General Virology, 101(6), 2020, https://doi.org/10.1099/jgv.0.001414. | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
その他のタイトル | ||||||
その他のタイトル | ヒトBST-2/tetherinはフニンウイルスの宿主細胞からの放出を阻害し、その阻害作用はウイルス核タンパク質により部分的に相殺される。 | |||||
出版者 | ||||||
出版者 | Microbiology Society | |||||
関係URI | ||||||
識別子タイプ | HDL | |||||
関連識別子 | http://hdl.handle.net/10069/40270 | |||||
学位名 | ||||||
学位名 | 博士(医学) | |||||
学位授与機関 | ||||||
学位授与機関識別子Scheme | kakenhi | |||||
学位授与機関識別子 | 17301 | |||||
学位授与機関名 | Nagasaki University (長崎大学) | |||||
学位授与年月日 | ||||||
学位授与年月日 | 2020-09-18 | |||||
学位授与番号 | ||||||
学位授与番号 | 甲医歯薬第1266号 | |||||
学位の種類 | ||||||
課程博士 | ||||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Nagasaki University (長崎大学), 博士(医学) (2020-09-18) |