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Development and Evaluation of an in-House IgM-Capture ELISA for the Detection of Chikungunya and Its Application to a Dengue Outbreak Situation in Kenya in 2013
http://hdl.handle.net/10069/35898
http://hdl.handle.net/10069/358987b944655-90ca-4eb8-bdee-723a57f2b9db
名前 / ファイル | ライセンス | アクション |
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JID68_410.pdf (333.9 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2015-10-21 | |||||
タイトル | ||||||
タイトル | Development and Evaluation of an in-House IgM-Capture ELISA for the Detection of Chikungunya and Its Application to a Dengue Outbreak Situation in Kenya in 2013 | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Chikungunya virus | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Focus reduction neutralization test | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | IgM-capture ELISA | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Kenya | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Wasonga, Caroline
× Wasonga, Caroline× Inoue, Shingo× Kimotho, James× Morita, Kouichi× Ongus, Juliette× Sang, Rosemary× Musila, Lillian |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Chikungunya (CHIK) is a mosquito-borne viral disease. In the 2004 CHIK outbreak in Kenya, diagnosis was delayed because of the lack of accurate diagnostics. Therefore, this study aimed to develop and evaluate an in-house IgM-capture enzyme linked immunosorbent assay (ELISA) (in-house ELISA) for the detection of chikungunya virus (CHIKV) infections. Anti-CHIKV antibodies were raised in rabbits, purified and conjugated to horseradish peroxidase. These anti-CHIKV antibodies and cell-culture derived antigen were used to develop the ELISA. To validate the in-house ELISA, 148 patient sera from the 2005 Comoros CHIK outbreak were tested with centers for disease control and prevention (CDC) IgM-capture ELISA (CDC ELISA) and focus reduction neutralization test (FRNT) as reference assays. The in-house ELISA had a sensitivity of 97.6% and specificity of 81.3% compared to the CDC ELISA and a sensitivity of 91.1% and specificity of 96.7% compared to FRNT. Furthermore, 254 clinically suspected dengue patient samples from Eastern Kenya, collected in 2013, were tested for CHIKV IgM using the in-house ELISA. Out of the 254 samples, 26 (10.2%) were IgM positive, and of these 26 samples, 17 were further analyzed by FRNT and 14 (82.4%) were positive. The in-house ELISA was able to diagnose CHIKV infection among suspected dengue cases in the 2013 outbreak. | |||||
書誌情報 |
Japanese Journal of Infectious Diseases 巻 68, 号 5, p. 410-414, 発行日 2015-09-19 |
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出版者 | ||||||
出版者 | 国立感染症研究所 | |||||
出版者別言語 | ||||||
National Institute of Infectious Diseases | ||||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 13446304 | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 18842836 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.7883/yoken.JJID.2014.482 | |||||
権利 | ||||||
権利情報 | c 2015 National Institute of Infectious Diseases | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Japanese Journal of Infectious Diseases, 68(5), pp.410-414; 2015 |