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Vascularized subcutaneous human liver tissue from engineered hepatocyte/fibroblast sheets in mice
http://hdl.handle.net/10069/36001
http://hdl.handle.net/10069/360011a408110-197c-49e9-b8d7-e4b4b70e7d17
名前 / ファイル | ライセンス | アクション |
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Biomaterials65_66.pdf (871.4 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2017-11-01 | |||||
タイトル | ||||||
タイトル | Vascularized subcutaneous human liver tissue from engineered hepatocyte/fibroblast sheets in mice | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Cell sheet | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Fibroblast | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Hepatocyte transplantation | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Human primary hepatocyte | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Tissue engineering | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Vascularization | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Sakai, Yusuke
× Sakai, Yusuke× Yamanouchi, Kosho× Ohashi, Kazuo× Koike, Makiko× Utoh, Rie× Hasegawa, Hideko× Muraoka, Izumi× Suematsu, Takashi× Soyama, Akihiko× Hidaka, Masaaki× Takatsuki, Mitsuhisa× Kuroki, Tamotsu× Eguchi, Susumu |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Subcutaneous liver tissue engineering is an attractive and minimally invasive approach used to curative treat hepatic failure and inherited liver diseases. However, graft failure occurs frequently due to insufficient infiltration of blood vessels (neoangiogenesis), while the maintenance of hepatocyte phenotype and function requires invivo development of the complex cellular organization of the hepatic lobule. Here we describe a subcutaneous human liver construction allowing for rapidly vascularized grafts by transplanting engineered cellular sheets consisting of human primary hepatocytes adhered onto a fibroblast layer. The engineered hepatocyte/fibroblast sheets (EHFSs) showed superior expression levels of vascularization-associated growth factors (vascular endothelial growth factor, transforming growth factor beta 1, and hepatocyte growth factor) invitro. EHFSs developed into vascularized subcutaneous human liver tissues contained glycogen stores, synthesized coagulation factor IX, and showed significantly higher synthesis rates of liver-specific proteins (albumin and alpha 1 anti-trypsin) invivo than tissues from hepatocyte-only sheets. The present study describes a new approach for vascularized human liver organogenesis under mouse skin. This approach could prove valuable for establishing novel cell therapies for liver diseases. | |||||
書誌情報 |
Biomaterials 巻 65, p. 66-75, 発行日 2015-10 |
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出版者 | ||||||
出版者 | Elsevier BV | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 01429612 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1016/j.biomaterials.2015.06.046 | |||||
権利 | ||||||
権利情報 | c 2015 Elsevier Ltd. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Biomaterials, 65, pp.66-75; 2015 |