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Keratinocyte Growth Factor Gene Electroporation into Skeletal Muscle as a Novel Gene Therapeutic Approach for Elastase-Induced Pulmonary Emphysema in Mice
http://hdl.handle.net/10069/36046
http://hdl.handle.net/10069/36046d6c3a0fb-5b18-4f16-999c-02aa5a564eca
名前 / ファイル | ライセンス | アクション |
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ISYK787_Tobinaga.pdf (2.3 MB)
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Item type | 学位論文 / Thesis or Dissertation(1) | |||||
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公開日 | 2015-12-14 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Keratinocyte Growth Factor Gene Electroporation into Skeletal Muscle as a Novel Gene Therapeutic Approach for Elastase-Induced Pulmonary Emphysema in Mice | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Elastase | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Electroporation | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Emphysema | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Gene therapy | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Keratinocyte growth factor | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||
資源タイプ | doctoral thesis | |||||
アクセス権 | ||||||
アクセス権 | open access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
著者 |
飛永, 修一
× 飛永, 修一 |
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著者別名 | ||||||
姓名 | Tobinaga, Shuichi | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Pulmonary emphysema is a progressive disease with airspace destruction and an effective therapy is needed. Keratinocyte growth factor (KGF) promotes pulmonary epithelial proliferation and has the potential to induce lung regeneration. The aim of this study was to determine the possibility of using KGF gene therapy for treatment of a mouse emphysema model induced by porcine pancreatic elastase (PPE). Eight-week-old BALB/c male mice treated with intra-tracheal PPE administration were transfected with 80 μg of a recombinant human KGF (rhKGF)-expressing FLAG-CMV14 plasmid (pKGF-FLAG gene), or with the pFLAG gene expressing plasmid as a control, into the quadriceps muscle by electroporation. In the lung, the expression of proliferating cell nuclear antigen (PCNA) was augmented, and surfactant protein A (SP-A) and KGF receptor (KGFR) were co-expressed in PCNA-positive cells. Moreover, endogenous KGF and KGFR gene expression increased significantly by pKGF-FLAG gene transfection. Arterial blood gas analysis revealed that the PaO2 level was not significantly reduced on day 14 after PPE instillation with pKGF-FLAG gene transfection compared to that of normal mice. These results indicated that KGF gene therapy with electroporation stimulated lung epithelial proliferation and protected depression of pulmonary function in a mouse emphysema model, suggesting a possible method of treating pulmonary emphysema. | |||||
内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 長崎大学学位論文 学位記番号:博(医歯薬)甲第787号 学位授与年月日:平成27年8月5日 | |||||
内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Author: Shuichi Tobinaga, Keitaro Matsumoto, Takeshi Nagayasu, Katsuro Furukawa, Takafumi Abo, Naoya Yamasaki, Tomoshi Tsuchiya, Takuro Miyazaki, Takehiko Koji | |||||
内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Citation: Acta Histochemica et Cytochemica, 48(3), pp.83-94; 2015 | |||||
書誌情報 |
Acta Histochemica et Cytochemica 巻 48, 号 3, p. 83-94, 発行日 2015-08-05 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 00445991 | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 13475800 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1267/ahc.15004 | |||||
権利 | ||||||
権利情報 | c 2015 The Japan Society of Histochemistry and Cytochemistry | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
その他のタイトル | ||||||
その他のタイトル | マウス肺気腫モデルに対するkeratinocyte growth factor 発現プラスミドを用いた新たな遺伝子治療手段の検討 | |||||
出版者 | ||||||
出版者 | 日本組織細胞化学会 | |||||
関係URI | ||||||
識別子タイプ | HDL | |||||
関連識別子 | http://hdl.handle.net/10069/35945 | |||||
学位名 | ||||||
学位名 | 博士(医学) | |||||
学位授与機関 | ||||||
学位授与機関識別子Scheme | kakenhi | |||||
学位授与機関識別子 | 17301 | |||||
学位授与機関名 | Nagasaki University (長崎大学) | |||||
学位授与年月日 | ||||||
学位授与年月日 | 2015-08-05 | |||||
学位授与番号 | ||||||
学位授与番号 | 甲医歯薬第787号 | |||||
学位の種類 | ||||||
課程博士 | ||||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Nagasaki University (長崎大学), 博士(医学) (2015-08-05) |