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When BMMs are stimulated with receptor activator of NF-κB ligand (RANKL) before TNF-α stimulation, pit-forming osteoclasts are differentiated. However, the details of the effect of RANKL pretreatment on the pit-forming osteoclast differentiation by TNF-α have not been established. The aim of this study is to examine the condition of RANKL pretreatment for differentiation of pit-forming osteoclasts induced by TNF-α.\nMurine BMMs were stimulated with various concentrations of RANKL for 24 h in the presence of M-CSF, then the medium was changed and TNF-α was added. Osteoclasts and pits formation were examined. Osteoprotegerin (OPG), decoy receptor of RANKL, was added to the culture to examine the necessity of co-existing RANKL with TNF-α on the formation of pit-forming osteoclasts. 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RANKL pretreatment plays an important role in the differentiation of pit-forming osteoclasts induced by TNF-α on murine bone marrow macrophages
http://hdl.handle.net/10069/36049
http://hdl.handle.net/10069/360498639e152-b196-4ac2-9ebf-54b685a7988c
名前 / ファイル | ライセンス | アクション |
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ISYK789_Yamashita.pdf (1.7 MB)
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Item type | 学位論文 / Thesis or Dissertation(1) | |||||
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公開日 | 2015-12-14 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | RANKL pretreatment plays an important role in the differentiation of pit-forming osteoclasts induced by TNF-α on murine bone marrow macrophages | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Receptor activator of NF-κB ligand | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Tumour necrosis factor-alpha | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Osteoclast | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Resorbing activity | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||
資源タイプ | doctoral thesis | |||||
アクセス権 | ||||||
アクセス権 | open access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
著者 |
山下, 恭德
× 山下, 恭德 |
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著者別名 | ||||||
姓名 | Yamashita, Yasunori | |||||
著者別名 | ||||||
姓名 | 山下, 恭徳 | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Background Osteoclasts differentiated from bone marrow macrophages (BMMs) induced by TNF-α alone do not have resorbing activity. When BMMs are stimulated with receptor activator of NF-κB ligand (RANKL) before TNF-α stimulation, pit-forming osteoclasts are differentiated. However, the details of the effect of RANKL pretreatment on the pit-forming osteoclast differentiation by TNF-α have not been established. The aim of this study is to examine the condition of RANKL pretreatment for differentiation of pit-forming osteoclasts induced by TNF-α. Murine BMMs were stimulated with various concentrations of RANKL for 24 h in the presence of M-CSF, then the medium was changed and TNF-α was added. Osteoclasts and pits formation were examined. Osteoprotegerin (OPG), decoy receptor of RANKL, was added to the culture to examine the necessity of co-existing RANKL with TNF-α on the formation of pit-forming osteoclasts. To investigate the influence of RANKL of sufficient concentration as pretreatment for pit-forming osteoclast formation by TNF-α, dose- and time-dependent changes of osteoclast formation were checked. Results The pit formation by osteoclasts in response to TNF-α required 10 ng/mL RANKL pretreatment. Stimulation with this concentration of RANKL led to the differentiation of mature osteoclasts in the 72 h culture. The pit formation was not inhibited by the OPG. Conclusion These results suggested that the concentration of RANKL pretreatment, which also alone can differentiate BMMs into osteoclasts, may be important in the differentiation of pit-forming osteoclasts by TNF-α. In addition, the effects of TNF-α after RANKL treatment might be independent of RANKL. |
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内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 長崎大学学位論文 学位記番号:博(医歯薬)甲第789号 学位授与年月日:平成27年8月5日 | |||||
内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Author: Yasunori Yamashita, Takashi Ukai, Hirotaka Nakamura, Yasunori Yoshinaga, Hiroki Kobayashi, Yuzo Takamori, Satoshi Noguchi, Atsutoshi Yoshimura, Yoshitaka Hara | |||||
内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Citation: Archives of Oral Biology, 60(9), pp.1273-1282; 2015 | |||||
書誌情報 |
Archives of Oral Biology 巻 60, 号 9, p. 1273-1282, 発行日 2015-08-05 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 00039969 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1016/j.archoralbio.2015.06.002 | |||||
権利 | ||||||
権利情報 | c 2015 Elsevier Ltd. | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
その他のタイトル | ||||||
その他のタイトル | RANKL 前刺激はマウス骨髄マクロファージにおいてTNF-α 刺激による吸収活性を持つ破骨細胞の分化に重要な役割を持つ | |||||
出版者 | ||||||
出版者 | Elsevier Ltd. | |||||
関係URI | ||||||
識別子タイプ | HDL | |||||
関連識別子 | http://hdl.handle.net/10069/35947 | |||||
学位名 | ||||||
学位名 | 博士(歯学) | |||||
学位授与機関 | ||||||
学位授与機関識別子Scheme | kakenhi | |||||
学位授与機関識別子 | 17301 | |||||
学位授与機関名 | Nagasaki University (長崎大学) | |||||
学位授与年月日 | ||||||
学位授与年月日 | 2015-08-05 | |||||
学位授与番号 | ||||||
学位授与番号 | 甲医歯薬第789号 | |||||
学位の種類 | ||||||
課程博士 | ||||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Nagasaki University (長崎大学), 博士(歯学) (2015-08-05) |