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Development of multiplex serological assay for the detection of human African trypanosomiasis
http://hdl.handle.net/10069/36089
http://hdl.handle.net/10069/36089fefd1adf-d15d-46ec-8c19-b8b3d12f9ef1
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
ParInt65_121.pdf (722.0 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2016-01-07 | |||||
| タイトル | ||||||
| タイトル | Development of multiplex serological assay for the detection of human African trypanosomiasis | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Human African trypanosomiasis | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Multiplex assay | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Recombinant antigens | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Sero-diagnosis | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Sero-surveillance | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 著者 |
Nzou, Samson Muuo
× Nzou, Samson Muuo× Fujii, Yoshito× Miura, Masashi× Mwau, Matilu× Mwangi, Anne Wanjiru× Itoh, Makoto× Salam, Md. Abdus× Hamano, Shinjiro× Hirayama, Kenji× Kaneko, Satoshi |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Human African trypanosomiasis (HAT) is a disease caused by Kinetoplastid infection. Serological tests are useful for epidemiological surveillance. The aim of this study was to develop a multiplex serological assay for HAT to assess the diagnostic value of selected HAT antigens for sero-epidemiological surveillance.We cloned loci encoding eight antigens from Trypanosoma brucei gambiense, expressed the genes in bacterial systems, and purified the resulting proteins. Antigens were subjected to Luminex multiplex assays using sera from HAT and VL patients to assess the antigens' immunodiagnostic potential. Among T. b. gambiense antigens, the 64-kDa and 65-kDa invariant surface glycoproteins (ISGs) and flagellar calcium binding protein (FCaBP) had high sensitivity for sera from T. b. gambiense patients, yielding AUC values of 0.871, 0.737 and 0.858 respectively in receiver operating characteristics (ROC) analysis. The ISG64, ISG65, and FCaBP antigens were partially cross-reactive to sera from Trypanosoma brucei rhodesiense patients. The GM6 antigen was cross-reactive to sera from T. b. rhodesiense patients as well as to sera from VL patients. Furthermore, heterogeneous antibody responses to each individual HAT antigen were observed. Testing for multiple HAT antigens in the same panel allowed specific and sensitive detection. Our results demonstrate the utility of applying multiplex assays for development and evaluation of HAT antigens for use in sero-epidemiological surveillance. | |||||
| 書誌情報 |
Parasitology International 巻 65, 号 2, p. 121-127, 発行日 2016-04 |
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| 出版者 | ||||||
| 出版者 | Elsevier Ireland Ltd | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 13835769 | |||||
| DOI | ||||||
| 関連タイプ | isIdenticalTo | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1016/j.parint.2015.10.008 | |||||
| 権利 | ||||||
| 権利情報 | c 2015 The Authors. Published by Elsevier Ireland Ltd. This is an open access article under the CC BY-NC-ND license(http://creativecommons.org/licenses/by-nc-nd/4.0/). | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| 引用 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Parasitology International, 65(2), pp.121-127; 2016 | |||||
