WEKO3
アイテム
{"_buckets": {"deposit": "8eca1fac-9369-462c-a933-1becd1d7c9ec"}, "_deposit": {"created_by": 2, "id": "5141", "owners": [2], "pid": {"revision_id": 0, "type": "depid", "value": "5141"}, "status": "published"}, "_oai": {"id": "oai:nagasaki-u.repo.nii.ac.jp:00005141", "sets": ["74"]}, "author_link": ["21274", "21281", "21276", "21280", "21275", "21278", "21273", "21282", "21283", "21272", "21277", "21279"], "item_2_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2013-12-04", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "12", "bibliographicPageStart": "e81191", "bibliographicVolumeNumber": "8", "bibliographic_titles": [{"bibliographic_title": "PLoS ONE"}]}]}, "item_2_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "99mTc-HYNIC-annexin A5 can be considered as a benchmark in the field of apoptosis imaging. However, 99mTc-HYNIC-annexin A5 has characteristics of high uptake and long retention in non-target tissues such as kidney and liver. To minimize this problem, we developed a novel 99mTc-labeled annexin A5 using a bis(hydroxamamide) derivative [C3(BHam)2] as a bifunctional chelating agent, and evaluated its usefulness as an imaging agent for detecting apoptosis. The amino group of C3(BHam)2 was converted to a maleimide group, and was coupled to thiol groups of annexin A5 pretreated with 2-iminothiolane. 99mTc labeling was performed by a ligand exchange reaction with 99mTc-glucoheptonate. Biodistribution experiments for both 99mTc-C3(BHam)2-annexin A5 and 99mTc-HYNIC-annexin A5 were performed in normal mice. In addition, in tumor-bearing mice, the relationship between the therapeutic effects of chemotherapy (5-FU) and the tumor accumulation of 99mTc-C 3(BHam)2-annexin A5 just after the first treatment of 5-FU was evaluated. 99mTc-C3(BHam)2-annexin A5 was prepared with a radiochemical purity of over 95%. In biodistribution experiments, 99mTc-C3(BHam)2-annexin A5 had a much lower kidney accumulation of radioactivity than 99mTc-HYNIC- annexin A5. In the organs for metabolism, such as liver and kidney, radioactivity after the injection of 99mTc-HYNIC-annexin A5 was residual for a long time. On the other hand, radioactivity after the injection of 99mTc-C3(BHam)2-annexin A5 gradually decreased. In therapeutic experiments, tumor growth in the mice treated with 5-FU was significantly inhibited. Accumulation of 99mTc-C 3(BHam)2-annexin A5 in tumors significantly increased after 5-FU treatment. The accumulation of radioactivity in tumor correlated positively with the counts of TUNEL-positive cells. These findings suggest that 99mTc-C3(BHam)2-annexin A5 may contribute to the efficient detection of apoptotic tumor response after chemotherapy.", "subitem_description_type": "Abstract"}]}, "item_2_description_63": {"attribute_name": "引用", "attribute_value_mlt": [{"subitem_description": "PLoS ONE, 8(12), e81191; 2013", "subitem_description_type": "Other"}]}, "item_2_publisher_33": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "Public Library of Science"}]}, "item_2_relation_12": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type": "isIdenticalTo", "subitem_relation_type_id": {"subitem_relation_type_id_text": "10.1371/journal.pone.0081191", "subitem_relation_type_select": "DOI"}}]}, "item_2_rights_13": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "c 2013 Ogawa et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited."}]}, "item_2_source_id_8": {"attribute_name": "EISSN", "attribute_value_mlt": [{"subitem_source_identifier": "19326203", "subitem_source_identifier_type": "ISSN"}]}, "item_2_version_type_16": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_970fb48d4fbd8a85", "subitem_version_type": "VoR"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Ogawa, Kazuma"}], "nameIdentifiers": [{"nameIdentifier": "21272", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Ohtsuki, Katsuichi"}], "nameIdentifiers": [{"nameIdentifier": "21273", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Shibata, Tomomi"}], "nameIdentifiers": [{"nameIdentifier": "21274", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Aoki, Miho"}], "nameIdentifiers": [{"nameIdentifier": "21275", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Nakayama, Morio"}], "nameIdentifiers": [{"nameIdentifier": "21276", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kitamura, Yoji"}], "nameIdentifiers": [{"nameIdentifier": "21277", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Ono, Masahiro"}], "nameIdentifiers": [{"nameIdentifier": "21278", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Ueda, Masashi"}], "nameIdentifiers": [{"nameIdentifier": "21279", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Doue, Tomoki"}], "nameIdentifiers": [{"nameIdentifier": "21280", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Onoguchi, Masahisa"}], "nameIdentifiers": [{"nameIdentifier": "21281", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Shiba, Kazuhiro"}], "nameIdentifiers": [{"nameIdentifier": "21282", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Odani, Akira"}], "nameIdentifiers": [{"nameIdentifier": "21283", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2020-12-21"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "PLoS8_81191.pdf", "filesize": [{"value": "1.1 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 1100000.0, "url": {"label": "PLoS8_81191.pdf", "url": "https://nagasaki-u.repo.nii.ac.jp/record/5141/files/PLoS8_81191.pdf"}, "version_id": "59eeb367-766b-460b-84c5-7485ba9031fe"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Development and Evaluation of a Novel 99mTc-Labeled Annexin A5 for Early Detection of Response to Chemotherapy", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Development and Evaluation of a Novel 99mTc-Labeled Annexin A5 for Early Detection of Response to Chemotherapy"}]}, "item_type_id": "2", "owner": "2", "path": ["74"], "permalink_uri": "http://hdl.handle.net/10069/34181", "pubdate": {"attribute_name": "公開日", "attribute_value": "2014-03-05"}, "publish_date": "2014-03-05", "publish_status": "0", "recid": "5141", "relation": {}, "relation_version_is_last": true, "title": ["Development and Evaluation of a Novel 99mTc-Labeled Annexin A5 for Early Detection of Response to Chemotherapy"], "weko_shared_id": -1}
Development and Evaluation of a Novel 99mTc-Labeled Annexin A5 for Early Detection of Response to Chemotherapy
http://hdl.handle.net/10069/34181
http://hdl.handle.net/10069/34181bcf208df-ca36-48c0-84bc-3f55e39a8874
名前 / ファイル | ライセンス | アクション |
---|---|---|
PLoS8_81191.pdf (1.1 MB)
|
|
Item type | 学術雑誌論文 / Journal Article(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2014-03-05 | |||||
タイトル | ||||||
タイトル | Development and Evaluation of a Novel 99mTc-Labeled Annexin A5 for Early Detection of Response to Chemotherapy | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Ogawa, Kazuma
× Ogawa, Kazuma× Ohtsuki, Katsuichi× Shibata, Tomomi× Aoki, Miho× Nakayama, Morio× Kitamura, Yoji× Ono, Masahiro× Ueda, Masashi× Doue, Tomoki× Onoguchi, Masahisa× Shiba, Kazuhiro× Odani, Akira |
|||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | 99mTc-HYNIC-annexin A5 can be considered as a benchmark in the field of apoptosis imaging. However, 99mTc-HYNIC-annexin A5 has characteristics of high uptake and long retention in non-target tissues such as kidney and liver. To minimize this problem, we developed a novel 99mTc-labeled annexin A5 using a bis(hydroxamamide) derivative [C3(BHam)2] as a bifunctional chelating agent, and evaluated its usefulness as an imaging agent for detecting apoptosis. The amino group of C3(BHam)2 was converted to a maleimide group, and was coupled to thiol groups of annexin A5 pretreated with 2-iminothiolane. 99mTc labeling was performed by a ligand exchange reaction with 99mTc-glucoheptonate. Biodistribution experiments for both 99mTc-C3(BHam)2-annexin A5 and 99mTc-HYNIC-annexin A5 were performed in normal mice. In addition, in tumor-bearing mice, the relationship between the therapeutic effects of chemotherapy (5-FU) and the tumor accumulation of 99mTc-C 3(BHam)2-annexin A5 just after the first treatment of 5-FU was evaluated. 99mTc-C3(BHam)2-annexin A5 was prepared with a radiochemical purity of over 95%. In biodistribution experiments, 99mTc-C3(BHam)2-annexin A5 had a much lower kidney accumulation of radioactivity than 99mTc-HYNIC- annexin A5. In the organs for metabolism, such as liver and kidney, radioactivity after the injection of 99mTc-HYNIC-annexin A5 was residual for a long time. On the other hand, radioactivity after the injection of 99mTc-C3(BHam)2-annexin A5 gradually decreased. In therapeutic experiments, tumor growth in the mice treated with 5-FU was significantly inhibited. Accumulation of 99mTc-C 3(BHam)2-annexin A5 in tumors significantly increased after 5-FU treatment. The accumulation of radioactivity in tumor correlated positively with the counts of TUNEL-positive cells. These findings suggest that 99mTc-C3(BHam)2-annexin A5 may contribute to the efficient detection of apoptotic tumor response after chemotherapy. | |||||
書誌情報 |
PLoS ONE 巻 8, 号 12, p. e81191, 発行日 2013-12-04 |
|||||
出版者 | ||||||
出版者 | Public Library of Science | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 19326203 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1371/journal.pone.0081191 | |||||
権利 | ||||||
権利情報 | c 2013 Ogawa et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | PLoS ONE, 8(12), e81191; 2013 |