WEKO3
アイテム
{"_buckets": {"deposit": "b1f792a8-17eb-4d64-9476-149282f92f77"}, "_deposit": {"created_by": 2, "id": "5219", "owners": [2], "pid": {"revision_id": 0, "type": "depid", "value": "5219"}, "status": "published"}, "_oai": {"id": "oai:nagasaki-u.repo.nii.ac.jp:00005219", "sets": ["11"]}, "author_link": ["21787", "21784", "21781", "21785", "21780", "21786", "21788", "21782", "21783"], "item_2_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2014-02-01", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "1", "bibliographicPageStart": "54", "bibliographicVolumeNumber": "14", "bibliographic_titles": [{"bibliographic_title": "BMC Infectious Diseases"}]}]}, "item_2_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "Background: The rapid identification of acid-fast bacilli recovered from patient specimens as Mycobacterium tuberculosis complex (MTC) is critically important for accurate diagnosis and treatment. A thin-layer immunochromatographic (TLC) assay using anti-MPB64 or anti-MPT64 monoclonal antibodies was developed to discriminate between MTC and non-tuberculosis mycobacteria (NTM). Capilia TB-Neo, which is the improved version of Capilia TB, is recently developed and needs to be evaluated.Methods: Capilia TB-Neo was evaluated by using reference strains including 96 Mycobacterium species (4 MTC and 92 NTM) and 3 other bacterial genera, and clinical isolates (500 MTC and 90 NTM isolates). M. tuberculosis isolates tested negative by Capilia TB-Neo were sequenced for mpt64 gene.Results: Capilia TB-Neo showed 100% agreement to a subset of reference strains. Non-specific reaction to M. marinum was not observed. The sensitivity and specificity of Capilia TB-Neo to the clinical isolates were 99.4% (99.6% for M. tuberculosis, excluding M. bovis BCG) for clinical MTC isolates and 100% for NTM isolates tested, respectively. Two M. tuberculosis isolates tested negative by Capilia TB-Neo: one harbored a 63-bp deletion in the mpt64 gene and the other possessed a 3,659-bp deletion from Rv1977 to Rv1981c, a region including the entire mpt64 gene.Conclusions: Capilia TB-Neo is a simple, rapid and highly sensitive test for identifying MTC, and showed better specificity than Capilia TB. However, Capilia TB-Neo still showed false-negative results with mpt64 mutations. The limitation should be recognized for clinical use.", "subitem_description_type": "Abstract"}]}, "item_2_description_63": {"attribute_name": "引用", "attribute_value_mlt": [{"subitem_description": "BMC Infectious Diseases, 14(1), 54; 2014", "subitem_description_type": "Other"}]}, "item_2_publisher_33": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "BioMed Central"}]}, "item_2_relation_12": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type": "isIdenticalTo", "subitem_relation_type_id": {"subitem_relation_type_id_text": "10.1186/1471-2334-14-54", "subitem_relation_type_select": "DOI"}}]}, "item_2_rights_13": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "c 2014 Chikamatsu et al.; licensee BioMed Central Ltd."}, {"subitem_rights": "This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited."}]}, "item_2_source_id_7": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "14712334", "subitem_source_identifier_type": "ISSN"}]}, "item_2_version_type_16": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_970fb48d4fbd8a85", "subitem_version_type": "VoR"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Chikamatsu, Kinuyo"}], "nameIdentifiers": [{"nameIdentifier": "21780", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Aono, Akio"}], "nameIdentifiers": [{"nameIdentifier": "21781", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Yamada, Hiroyuki"}], "nameIdentifiers": [{"nameIdentifier": "21782", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Sugamoto, Tetsuhiro"}], "nameIdentifiers": [{"nameIdentifier": "21783", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kato, Tomoko"}], "nameIdentifiers": [{"nameIdentifier": "21784", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kazumi, Yuko"}], "nameIdentifiers": [{"nameIdentifier": "21785", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Tamai, Kiyoko"}], "nameIdentifiers": [{"nameIdentifier": "21786", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Yanagisawa, Hideji"}], "nameIdentifiers": [{"nameIdentifier": "21787", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Mitarai, Satoshi"}], "nameIdentifiers": [{"nameIdentifier": "21788", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2020-12-21"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "BMCID14_54.pdf", "filesize": [{"value": "263.7 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 263700.0, "url": {"label": "BMCID14_54.pdf", "url": "https://nagasaki-u.repo.nii.ac.jp/record/5219/files/BMCID14_54.pdf"}, "version_id": "211c788a-c44d-4623-bbbc-6819c8ee323a"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "Capilia TB-Neo", "subitem_subject_scheme": "Other"}, {"subitem_subject": "mpt64 gene", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Mycobacterium tuberculosis complex identification", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Comparative evaluation of three immunochromatographic identification tests for culture confirmation of Mycobacterium tuberculosis complex", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Comparative evaluation of three immunochromatographic identification tests for culture confirmation of Mycobacterium tuberculosis complex"}]}, "item_type_id": "2", "owner": "2", "path": ["11"], "permalink_uri": "http://hdl.handle.net/10069/34271", "pubdate": {"attribute_name": "公開日", "attribute_value": "2014-04-15"}, "publish_date": "2014-04-15", "publish_status": "0", "recid": "5219", "relation": {}, "relation_version_is_last": true, "title": ["Comparative evaluation of three immunochromatographic identification tests for culture confirmation of Mycobacterium tuberculosis complex"], "weko_shared_id": -1}
Comparative evaluation of three immunochromatographic identification tests for culture confirmation of Mycobacterium tuberculosis complex
http://hdl.handle.net/10069/34271
http://hdl.handle.net/10069/34271d1e9c89c-c340-4e3c-a48c-0c6a8889b78e
名前 / ファイル | ライセンス | アクション |
---|---|---|
BMCID14_54.pdf (263.7 kB)
|
|
Item type | 学術雑誌論文 / Journal Article(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2014-04-15 | |||||
タイトル | ||||||
タイトル | Comparative evaluation of three immunochromatographic identification tests for culture confirmation of Mycobacterium tuberculosis complex | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Capilia TB-Neo | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | mpt64 gene | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Mycobacterium tuberculosis complex identification | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Chikamatsu, Kinuyo
× Chikamatsu, Kinuyo× Aono, Akio× Yamada, Hiroyuki× Sugamoto, Tetsuhiro× Kato, Tomoko× Kazumi, Yuko× Tamai, Kiyoko× Yanagisawa, Hideji× Mitarai, Satoshi |
|||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Background: The rapid identification of acid-fast bacilli recovered from patient specimens as Mycobacterium tuberculosis complex (MTC) is critically important for accurate diagnosis and treatment. A thin-layer immunochromatographic (TLC) assay using anti-MPB64 or anti-MPT64 monoclonal antibodies was developed to discriminate between MTC and non-tuberculosis mycobacteria (NTM). Capilia TB-Neo, which is the improved version of Capilia TB, is recently developed and needs to be evaluated.Methods: Capilia TB-Neo was evaluated by using reference strains including 96 Mycobacterium species (4 MTC and 92 NTM) and 3 other bacterial genera, and clinical isolates (500 MTC and 90 NTM isolates). M. tuberculosis isolates tested negative by Capilia TB-Neo were sequenced for mpt64 gene.Results: Capilia TB-Neo showed 100% agreement to a subset of reference strains. Non-specific reaction to M. marinum was not observed. The sensitivity and specificity of Capilia TB-Neo to the clinical isolates were 99.4% (99.6% for M. tuberculosis, excluding M. bovis BCG) for clinical MTC isolates and 100% for NTM isolates tested, respectively. Two M. tuberculosis isolates tested negative by Capilia TB-Neo: one harbored a 63-bp deletion in the mpt64 gene and the other possessed a 3,659-bp deletion from Rv1977 to Rv1981c, a region including the entire mpt64 gene.Conclusions: Capilia TB-Neo is a simple, rapid and highly sensitive test for identifying MTC, and showed better specificity than Capilia TB. However, Capilia TB-Neo still showed false-negative results with mpt64 mutations. The limitation should be recognized for clinical use. | |||||
書誌情報 |
BMC Infectious Diseases 巻 14, 号 1, p. 54, 発行日 2014-02-01 |
|||||
出版者 | ||||||
出版者 | BioMed Central | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 14712334 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1186/1471-2334-14-54 | |||||
権利 | ||||||
権利情報 | c 2014 Chikamatsu et al.; licensee BioMed Central Ltd. | |||||
権利 | ||||||
権利情報 | This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | BMC Infectious Diseases, 14(1), 54; 2014 |