WEKO3
アイテム
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Identification of an O-antigen chain length regulator, WzzP, in Porphyromonas gingivalis
http://hdl.handle.net/10069/34411
http://hdl.handle.net/10069/344114a8a9e29-92c6-464b-a2ae-0d6ad12e688f
名前 / ファイル | ライセンス | アクション |
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MicOpe2_383.pdf (2.1 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2014-05-27 | |||||
タイトル | ||||||
タイトル | Identification of an O-antigen chain length regulator, WzzP, in Porphyromonas gingivalis | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | LPS biosynthesis | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Periodontal pathogen | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | WbaP protein | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Wzx protein | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Wzz protein | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Shoji, Mikio
× Shoji, Mikio× Yukitake, Hideharu× Sato, Keiko× Shibata, Yasuko× Naito, Mariko× Aduse-Opoku, Joseph× Abiko, Yoshimitsu× Curtis, Michael A.× Nakayama, Koji |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | The periodontal pathogen Porphyromonas gingivalis has two different lipopolysaccharides (LPSs) designated O-LPS and A-LPS, which are a conventional O-antigen polysaccharide and an anionic polysaccharide that are both linked to lipid A-cores, respectively. However, the precise mechanisms of LPS biosynthesis remain to be determined. In this study, we isolated a pigment-less mutant by transposon mutagenesis and identified that the transposon was inserted into the coding sequence PGN_2005, which encodes a hypothetical protein of P. gingivalis ATCC 33277. We found that (i) LPSs purified from the PGN_2005 mutant were shorter than those of the wild type; (ii) the PGN_2005 protein was located in the inner membrane fraction; and (iii) the PGN_2005 gene conferred Wzz activity upon an Escherichia coli wzz mutant. These results indicate that the PGN_2005 protein, which was designated WzzP, is a functional homolog of the Wzz protein in P. gingivalis. Comparison of amino acid sequences among WzzP and conventional Wzz proteins indicated that WzzP had an additional fragment at the C-terminal region. In addition, we determined that the PGN_1896 and PGN_1233 proteins and the PGN_1033 protein appear to be WbaP homolog proteins and a Wzx homolog protein involved in LPS biosynthesis, respectively. | |||||
書誌情報 |
MicrobiologyOpen 巻 2, 号 3, p. 383-401, 発行日 2013-06 |
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出版者 | ||||||
出版者 | John Wiley and Sons Inc. | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 20458827 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1002/mbo3.84 | |||||
権利 | ||||||
権利情報 | c 2013 The Authors. Microbiology Open published by John Wiley & Sons Ltd. | |||||
権利 | ||||||
権利情報 | This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | MicrobiologyOpen, 2(3), pp.383-401; 2013 |