WEKO3
アイテム
{"_buckets": {"deposit": "82780c7c-5880-42f8-a43a-a77be1bc97a6"}, "_deposit": {"created_by": 2, "id": "5416", "owners": [2], "pid": {"revision_id": 0, "type": "depid", "value": "5416"}, "status": "published"}, "_oai": {"id": "oai:nagasaki-u.repo.nii.ac.jp:00005416", "sets": ["26"]}, "author_link": ["22942", "22946", "22950", "22939", "22952", "22944", "22945", "22948", "22951", "22947", "22953", "22949", "22954", "22943", "22941", "22940"], "item_2_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2014-01-27", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "1", "bibliographicPageStart": "e86455", "bibliographicVolumeNumber": "9", "bibliographic_titles": [{"bibliographic_title": "PLoS ONE"}]}]}, "item_2_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "Cyanovirin-N (CVN) potently inhibits human immunodeficiency virus type 1 (HIV-1) infection, but both cytotoxicity and immunogenicity have hindered the translation of this protein into a viable therapeutic. A molecular docking analysis suggested that up to 12 residues were involved in the interaction of the reverse parallel CVN dimer with the oligosaccharide targets, among which Leu-1 was the most prominent hot spot residue. This finding provided a possible explanation for the lack of anti-HIV-1 activity observed with N-terminal PEGylated CVN. Therefore, linker-CVN (LCVN) was designed as a CVN derivative with a flexible and hydrophilic linker (Gly4Ser)3 at the N-terminus. The N-terminal α-amine of LCVN was PEGylated to create 10 K PEG-aldehyde (ALD)-LCVN. LCVN and 10 K PEG-ALD-LCVN retained the specificity and affinity of CVN for high mannose N-glycans. Moreover, LCVN exhibited significant anti-HIV-1 activity with attenuated cytotoxicity in the HaCaT keratinocyte cell line and MT-4 T lymphocyte cell lines. 10 K PEG-ALD-LCVN also efficiently inactivated HIV-1 with remarkably decreased cytotoxicity and pronounced cell-to-cell fusion inhibitory activity in vitro. The linker-extended CVN and the mono-PEGylated derivative were determined to be promising candidates for the development of an anti-HIV-1 agent. This derivatization approach provided a model for the PEGylation of biologic candidates without introducing point mutations.", "subitem_description_type": "Abstract"}]}, "item_2_description_63": {"attribute_name": "引用", "attribute_value_mlt": [{"subitem_description": "PLoS ONE, 9(1), e86455; 2014", "subitem_description_type": "Other"}]}, "item_2_publisher_33": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "Public Library of Science"}]}, "item_2_relation_12": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type": "isIdenticalTo", "subitem_relation_type_id": {"subitem_relation_type_id_text": "10.1371/journal.pone.0086455", "subitem_relation_type_select": "DOI"}}]}, "item_2_rights_13": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "c 2014 Chen et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited."}]}, "item_2_source_id_8": {"attribute_name": "EISSN", "attribute_value_mlt": [{"subitem_source_identifier": "19326203", "subitem_source_identifier_type": "ISSN"}]}, "item_2_version_type_16": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_970fb48d4fbd8a85", "subitem_version_type": "VoR"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Chen, Jia"}], "nameIdentifiers": [{"nameIdentifier": "22939", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Huang, Dane"}], "nameIdentifiers": [{"nameIdentifier": "22940", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Chen, Wei"}], "nameIdentifiers": [{"nameIdentifier": "22941", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Guo, Chaowan"}], "nameIdentifiers": [{"nameIdentifier": "22942", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Wei, Bo"}], "nameIdentifiers": [{"nameIdentifier": "22943", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Wu, Chongchao"}], "nameIdentifiers": [{"nameIdentifier": "22944", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Peng, Zhou"}], "nameIdentifiers": [{"nameIdentifier": "22945", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Fan, Jun"}], "nameIdentifiers": [{"nameIdentifier": "22946", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Hou, Zhibo"}], "nameIdentifiers": [{"nameIdentifier": "22947", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Fang, Yongsheng"}], "nameIdentifiers": [{"nameIdentifier": "22948", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Wang, Yifei"}], "nameIdentifiers": [{"nameIdentifier": "22949", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kitazato, Kaio"}], "nameIdentifiers": [{"nameIdentifier": "22950", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Yu, Guoying"}], "nameIdentifiers": [{"nameIdentifier": "22951", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Zou, Chunbin"}], "nameIdentifiers": [{"nameIdentifier": "22952", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Qian, Chuiwen"}], "nameIdentifiers": [{"nameIdentifier": "22953", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Xiong, Sheng"}], "nameIdentifiers": [{"nameIdentifier": "22954", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2020-12-21"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "PLoS9_86455.pdf", "filesize": [{"value": "2.8 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 2800000.0, "url": {"label": "PLoS9_86455.pdf", "url": "https://nagasaki-u.repo.nii.ac.jp/record/5416/files/PLoS9_86455.pdf"}, "version_id": "fc5dbb43-ebe3-42c4-9c6b-644908e12008"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Linker-Extended Native Cyanovirin-N Facilitates PEGylation and Potently Inhibits HIV-1 by Targeting the Glycan Ligand", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Linker-Extended Native Cyanovirin-N Facilitates PEGylation and Potently Inhibits HIV-1 by Targeting the Glycan Ligand"}]}, "item_type_id": "2", "owner": "2", "path": ["26"], "permalink_uri": "http://hdl.handle.net/10069/34491", "pubdate": {"attribute_name": "公開日", "attribute_value": "2014-06-20"}, "publish_date": "2014-06-20", "publish_status": "0", "recid": "5416", "relation": {}, "relation_version_is_last": true, "title": ["Linker-Extended Native Cyanovirin-N Facilitates PEGylation and Potently Inhibits HIV-1 by Targeting the Glycan Ligand"], "weko_shared_id": -1}
Linker-Extended Native Cyanovirin-N Facilitates PEGylation and Potently Inhibits HIV-1 by Targeting the Glycan Ligand
http://hdl.handle.net/10069/34491
http://hdl.handle.net/10069/344913117a3ea-73d0-4615-be27-477362c47095
名前 / ファイル | ライセンス | アクション |
---|---|---|
PLoS9_86455.pdf (2.8 MB)
|
|
Item type | 学術雑誌論文 / Journal Article(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2014-06-20 | |||||
タイトル | ||||||
タイトル | Linker-Extended Native Cyanovirin-N Facilitates PEGylation and Potently Inhibits HIV-1 by Targeting the Glycan Ligand | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Chen, Jia
× Chen, Jia× Huang, Dane× Chen, Wei× Guo, Chaowan× Wei, Bo× Wu, Chongchao× Peng, Zhou× Fan, Jun× Hou, Zhibo× Fang, Yongsheng× Wang, Yifei× Kitazato, Kaio× Yu, Guoying× Zou, Chunbin× Qian, Chuiwen× Xiong, Sheng |
|||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Cyanovirin-N (CVN) potently inhibits human immunodeficiency virus type 1 (HIV-1) infection, but both cytotoxicity and immunogenicity have hindered the translation of this protein into a viable therapeutic. A molecular docking analysis suggested that up to 12 residues were involved in the interaction of the reverse parallel CVN dimer with the oligosaccharide targets, among which Leu-1 was the most prominent hot spot residue. This finding provided a possible explanation for the lack of anti-HIV-1 activity observed with N-terminal PEGylated CVN. Therefore, linker-CVN (LCVN) was designed as a CVN derivative with a flexible and hydrophilic linker (Gly4Ser)3 at the N-terminus. The N-terminal α-amine of LCVN was PEGylated to create 10 K PEG-aldehyde (ALD)-LCVN. LCVN and 10 K PEG-ALD-LCVN retained the specificity and affinity of CVN for high mannose N-glycans. Moreover, LCVN exhibited significant anti-HIV-1 activity with attenuated cytotoxicity in the HaCaT keratinocyte cell line and MT-4 T lymphocyte cell lines. 10 K PEG-ALD-LCVN also efficiently inactivated HIV-1 with remarkably decreased cytotoxicity and pronounced cell-to-cell fusion inhibitory activity in vitro. The linker-extended CVN and the mono-PEGylated derivative were determined to be promising candidates for the development of an anti-HIV-1 agent. This derivatization approach provided a model for the PEGylation of biologic candidates without introducing point mutations. | |||||
書誌情報 |
PLoS ONE 巻 9, 号 1, p. e86455, 発行日 2014-01-27 |
|||||
出版者 | ||||||
出版者 | Public Library of Science | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 19326203 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1371/journal.pone.0086455 | |||||
権利 | ||||||
権利情報 | c 2014 Chen et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | PLoS ONE, 9(1), e86455; 2014 |