WEKO3
アイテム
{"_buckets": {"deposit": "f0ee7c31-b37a-47e6-99fb-f6bf5bfa7459"}, "_deposit": {"created_by": 2, "id": "5612", "owners": [2], "pid": {"revision_id": 0, "type": "depid", "value": "5612"}, "status": "published"}, "_oai": {"id": "oai:nagasaki-u.repo.nii.ac.jp:00005612", "sets": ["346"]}, "author_link": ["23780", "23776", "23778", "23781", "23779", "23777", "23782"], "item_3_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2014-07", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "1", "bibliographicPageEnd": "6", "bibliographicPageStart": "1", "bibliographicVolumeNumber": "59", "bibliographic_titles": [{"bibliographic_title": "Acta medica Nagasakiensia"}]}]}, "item_3_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "Background.\nAlthough the development of a practicable culture method to maintain long-term hepatocyte function is highly desirable, hepatocytes lose their liver-specific functions rapidly on regular collagen-coated dishes. In this study, we evaluated the efficacy of a special cell culture dish coated with the temperature-responsive polymer poly(N-isopropylacrylamide) (PIPAAm), and observed the morphological and functional changes of hepatocytes on changing the amount of polymer.\nMethods.\nCulture plates with varying amounts of PIPAAm polymer (1.5~3.5 times that in the commercially available temperatureresponsive polymer-containing dish (UpCellR, CellSeed, Tokyo, Japan)) were prepared. All dishes were enhanced by adding a layer of rat tail collagen I at a dose of 600 μg/dish for 3 hours. Hepatocytes isolated from Sprague-Dawley rats were cultured in these static culture systems. Morphologic changes and liver-specific functions were evaluated.\nResults.\nOn day 7 of culture, spheroid formation of hepatocytes was observed in the high-polymer group, with the presence of glycogen and albumin in the spheroid. In the high-polymer group, the rate of albumin production was significantly higher than in the low-polymer group until day 14 of culture (P\u003c0.001).\nConclusion.\nThe spheroid formation of hepatocytes cultured in the presence of a high level of PIPAAm showed the long-term maintenance of liver-specific functions in vitro.", "subitem_description_type": "Abstract"}]}, "item_3_description_64": {"attribute_name": "引用", "attribute_value_mlt": [{"subitem_description": "Acta medica Nagasakiensia, 59(1), pp.1-6; 2014", "subitem_description_type": "Other"}]}, "item_3_publisher_33": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "Nagasaki University School of Medicine"}]}, "item_3_source_id_10": {"attribute_name": "書誌レコードID", "attribute_value_mlt": [{"subitem_source_identifier": "AA00508430", "subitem_source_identifier_type": "NCID"}]}, "item_3_source_id_7": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "00016055", "subitem_source_identifier_type": "ISSN"}]}, "item_3_text_62": {"attribute_name": "sortkey", "attribute_value_mlt": [{"subitem_text_value": "1"}]}, "item_3_text_63": {"attribute_name": "出版者別言語", "attribute_value_mlt": [{"subitem_text_value": "長崎大学医学部"}]}, "item_3_version_type_16": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_970fb48d4fbd8a85", "subitem_version_type": "VoR"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Tomonaga, Tetsuo"}], "nameIdentifiers": [{"nameIdentifier": "23776", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Soyama, Akihiko"}], "nameIdentifiers": [{"nameIdentifier": "23777", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Yamanouchi, Kosho"}], "nameIdentifiers": [{"nameIdentifier": "23778", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Takatsuki, Mitsuhisa"}], "nameIdentifiers": [{"nameIdentifier": "23779", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kuroki, Tamotsu"}], "nameIdentifiers": [{"nameIdentifier": "23780", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Koji, Takehiko"}], "nameIdentifiers": [{"nameIdentifier": "23781", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Eguchi, Susumu"}], "nameIdentifiers": [{"nameIdentifier": "23782", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2020-12-21"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "ActMed59_1.pdf", "filesize": [{"value": "1.1 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 1100000.0, "url": {"label": "ActMed59_1.pdf", "url": "https://nagasaki-u.repo.nii.ac.jp/record/5612/files/ActMed59_1.pdf"}, "version_id": "24ac3d85-1dc4-4882-8ec1-9ede2a9435db"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "Hepatocyte", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Temperature-responsive Surface", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Spheroid", "subitem_subject_scheme": "Other"}, {"subitem_subject": "PIPAAm", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Rat hepatocyte spheroids formed on temperature-responsive PIPAAm polymer-grafted surface maintain long-term differentiated hepatocyte function", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Rat hepatocyte spheroids formed on temperature-responsive PIPAAm polymer-grafted surface maintain long-term differentiated hepatocyte function"}]}, "item_type_id": "3", "owner": "2", "path": ["346"], "permalink_uri": "http://hdl.handle.net/10069/34694", "pubdate": {"attribute_name": "公開日", "attribute_value": "2014-09-09"}, "publish_date": "2014-09-09", "publish_status": "0", "recid": "5612", "relation": {}, "relation_version_is_last": true, "title": ["Rat hepatocyte spheroids formed on temperature-responsive PIPAAm polymer-grafted surface maintain long-term differentiated hepatocyte function"], "weko_shared_id": 2}
Rat hepatocyte spheroids formed on temperature-responsive PIPAAm polymer-grafted surface maintain long-term differentiated hepatocyte function
http://hdl.handle.net/10069/34694
http://hdl.handle.net/10069/346941d6ed7a2-15fa-4d86-8564-eaf81b80ee01
名前 / ファイル | ライセンス | アクション |
---|---|---|
ActMed59_1.pdf (1.1 MB)
|
|
Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2014-09-09 | |||||
タイトル | ||||||
タイトル | Rat hepatocyte spheroids formed on temperature-responsive PIPAAm polymer-grafted surface maintain long-term differentiated hepatocyte function | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Hepatocyte | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Temperature-responsive Surface | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Spheroid | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | PIPAAm | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
著者 |
Tomonaga, Tetsuo
× Tomonaga, Tetsuo× Soyama, Akihiko× Yamanouchi, Kosho× Takatsuki, Mitsuhisa× Kuroki, Tamotsu× Koji, Takehiko× Eguchi, Susumu |
|||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Background. Although the development of a practicable culture method to maintain long-term hepatocyte function is highly desirable, hepatocytes lose their liver-specific functions rapidly on regular collagen-coated dishes. In this study, we evaluated the efficacy of a special cell culture dish coated with the temperature-responsive polymer poly(N-isopropylacrylamide) (PIPAAm), and observed the morphological and functional changes of hepatocytes on changing the amount of polymer. Methods. Culture plates with varying amounts of PIPAAm polymer (1.5~3.5 times that in the commercially available temperatureresponsive polymer-containing dish (UpCellR, CellSeed, Tokyo, Japan)) were prepared. All dishes were enhanced by adding a layer of rat tail collagen I at a dose of 600 μg/dish for 3 hours. Hepatocytes isolated from Sprague-Dawley rats were cultured in these static culture systems. Morphologic changes and liver-specific functions were evaluated. Results. On day 7 of culture, spheroid formation of hepatocytes was observed in the high-polymer group, with the presence of glycogen and albumin in the spheroid. In the high-polymer group, the rate of albumin production was significantly higher than in the low-polymer group until day 14 of culture (P<0.001). Conclusion. The spheroid formation of hepatocytes cultured in the presence of a high level of PIPAAm showed the long-term maintenance of liver-specific functions in vitro. |
|||||
書誌情報 |
Acta medica Nagasakiensia 巻 59, 号 1, p. 1-6, 発行日 2014-07 |
|||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 00016055 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA00508430 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
出版者 | ||||||
出版者 | Nagasaki University School of Medicine | |||||
出版者別言語 | ||||||
長崎大学医学部 | ||||||
sortkey | ||||||
1 | ||||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Acta medica Nagasakiensia, 59(1), pp.1-6; 2014 |