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Serological Surveillance Development for Tropical Infectious Diseases Using Simultaneous Microsphere-Based Multiplex Assays and Finite Mixture Models
http://hdl.handle.net/10069/34755
http://hdl.handle.net/10069/347553cb1d1de-a8fb-4d4d-a006-a4c88f928ba5
名前 / ファイル | ライセンス | アクション |
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PLoSNeg8_3040.pdf (6.3 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2014-10-06 | |||||
タイトル | ||||||
タイトル | Serological Surveillance Development for Tropical Infectious Diseases Using Simultaneous Microsphere-Based Multiplex Assays and Finite Mixture Models | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Fujii, Yoshito
× Fujii, Yoshito× Kaneko, Satoshi× Nzou, Samson Muuo× Mwau, Matilu× Njenga, Sammy M.× Tanigawa, Chihiro× Kimotho, James× Mwangi, Anne Wanjiru× Kiche, Ibrahim× Matsumoto, Sohkichi× Niki, Mamiko× Osada-Oka, Mayuko× Ichinose, Yoshio× Inoue, Manabu× Itoh, Makoto× Tachibana, Hiroshi× Ishii, Kazunari× Tsuboi, Takafumi× Yoshida, Lay Myint× Mondal, Dinesh× Haque, Rashidul× Hamano, Shinjiro× Changoma, Mwatasa× Hoshi, Tomonori× Kamo, Ken-ichi× Karama, Mohamed× Miura, Masashi× Hirayama, Kenji |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Background:A strategy to combat infectious diseases, including neglected tropical diseases (NTDs), will depend on the development of reliable epidemiological surveillance methods. To establish a simple and practical seroprevalence detection system, we developed a microsphere-based multiplex immunoassay system and evaluated utility using samples obtained in Kenya.Methods:We developed a microsphere-based immuno-assay system to simultaneously measure the individual levels of plasma antibody (IgG) against 8 antigens derived from 6 pathogens: Entamoeba histolytica (C-IgL), Leishmania donovani (KRP42), Toxoplasma gondii (SAG1), Wuchereria bancrofti (SXP1), HIV (gag, gp120 and gp41), and Vibrio cholerae (cholera toxin). The assay system was validated using appropriate control samples. The assay system was applied for 3411 blood samples collected from the general population randomly selected from two health and demographic surveillance system (HDSS) cohorts in the coastal and western regions of Kenya. The immunoassay values distribution for each antigen was mathematically defined by a finite mixture model, and cut-off values were optimized.Findings:Sensitivities and specificities for each antigen ranged between 71 and 100%. Seroprevalences for each pathogen from the Kwale and Mbita HDSS sites (respectively) were as follows: HIV, 3.0% and 20.1%; L. donovani, 12.6% and 17.3%; E. histolytica, 12.8% and 16.6%; and T. gondii, 30.9% and 28.2%. Seroprevalences of W. bancrofti and V. cholerae showed relatively high figures, especially among children. The results might be affected by immunological cross reactions between W. bancrofti-SXP1 and other parasitic infections; and cholera toxin and the enterotoxigenic E. coli (ETEC), respectively.Interpretation:A microsphere-based multi-serological assay system can provide an opportunity to comprehensively grasp epidemiological features for NTDs. By adding pathogens and antigens of interest, optimized made-to-order high-quality programs can be established to utilize limited resources to effectively control NTDs in Africa. | |||||
書誌情報 |
PLoS Neglected Tropical Diseases 巻 8, 号 7, p. e3040, 発行日 2014-07-31 |
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出版者 | ||||||
出版者 | Public Library of Science | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 19352735 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1371/journal.pntd.0003040 | |||||
権利 | ||||||
権利情報 | c 2014 Fujii et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | PLoS Neglected Tropical Diseases, 8(7), e3040; 2014 |