WEKO3
アイテム
{"_buckets": {"deposit": "a07f3f26-310c-4c99-b6c3-e621d08b33da"}, "_deposit": {"created_by": 2, "id": "5726", "owners": [2], "pid": {"revision_id": 0, "type": "depid", "value": "5726"}, "status": "published"}, "_oai": {"id": "oai:nagasaki-u.repo.nii.ac.jp:00005726", "sets": ["9"]}, "author_link": ["24590", "24584", "24581", "24588", "24586", "24583", "24585", "24589", "24582", "24587"], "item_2_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2014-08-01", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "8", "bibliographicPageEnd": "1281", "bibliographicPageStart": "1274", "bibliographicVolumeNumber": "37", "bibliographic_titles": [{"bibliographic_title": "Biological and Pharmaceutical Bulletin"}]}]}, "item_2_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "We developed a novel small interfering RNA (siRNA) delivery system using a ternary complex with polyethyleneimine (PEI) and γ-polyglutamic acid (γ-PGA), which showed silencing effect and no cytotoxicity. The binary complexes of siRNA with PEI were approximately 73-102 nm in particle size and 45-52 mV in a-potential. The silencing effect of siRNA/PEI complexes increased with an increase of PEI, and siRNA/PEI complexes with a charge ratio greater than 16 showed significant luciferase knockdown in a mouse colon carcinoma cell line regularly expressing luciferase (Colon26/Luc cells). However, strong cytotoxicity and blood agglutination were observed in the siRNA/Lipofectamine complex and siRNA/PEI16 complex. Recharging cationic complexes with an anionic compound was reported to be a promising method for overcoming these toxicities. We therefore prepared ternary complexes of siRNA with PEI (charge ratio 16) by the addition of γ-PGA to reduce cytotoxicity and deliver siRNA. As expected, the cytotoxicity of the ternary complexes decreased with an increase of γ-PGA content, which decreased the a-potential of the complexes. A strong silencing effect comparable to siRNA/Lipofectamine complex was discovered in ternary complexes including γ-PGA with an anionic surface charge. The high incorporation of ternary complexes into Colon26/Luc cells was confirmed with fluorescence microcopy. Having achieved knockdown of an exogenously transfected gene, the ability of the complex to mediate knockdown of an endogenous housekeeping gene, glyceraldehyde 3-phosphate dehydrogenase (GAPDH), was assessed in B16-F10 cells. The ternary complex (siRNA/PEI16/γ-PGA12 complex) exhibited a significant GAPDH knockdown effect. Thus, we developed a useful siRNA delivery system.", "subitem_description_type": "Abstract"}]}, "item_2_description_63": {"attribute_name": "引用", "attribute_value_mlt": [{"subitem_description": "Biological and Pharmaceutical Bulletin, 37(8), pp.1274-1281; 2014", "subitem_description_type": "Other"}]}, "item_2_publisher_33": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "日本薬学会"}]}, "item_2_relation_12": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type": "isIdenticalTo", "subitem_relation_type_id": {"subitem_relation_type_id_text": "10.1248/bpb.b14-00058", "subitem_relation_type_select": "DOI"}}]}, "item_2_rights_13": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "c 2014 The Pharmaceutical Society of Japan"}]}, "item_2_source_id_7": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "09186158", "subitem_source_identifier_type": "ISSN"}]}, "item_2_source_id_8": {"attribute_name": "EISSN", "attribute_value_mlt": [{"subitem_source_identifier": "13475215", "subitem_source_identifier_type": "ISSN"}]}, "item_2_text_62": {"attribute_name": "出版者別言語", "attribute_value_mlt": [{"subitem_text_value": "Pharmaceutical Society of Japan"}]}, "item_2_version_type_16": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_970fb48d4fbd8a85", "subitem_version_type": "VoR"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Kodama, Yukinobu"}], "nameIdentifiers": [{"nameIdentifier": "24581", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Shiokawa, Yumi"}], "nameIdentifiers": [{"nameIdentifier": "24582", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Nakamura, Tadahiro"}], "nameIdentifiers": [{"nameIdentifier": "24583", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kurosaki, Tomoaki"}], "nameIdentifiers": [{"nameIdentifier": "24584", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Aki, Keisei"}], "nameIdentifiers": [{"nameIdentifier": "24585", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Nakagawa, Hiroo"}], "nameIdentifiers": [{"nameIdentifier": "24586", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Muro, Takahiro"}], "nameIdentifiers": [{"nameIdentifier": "24587", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kitahara, Takashi"}], "nameIdentifiers": [{"nameIdentifier": "24588", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Higuchi, Norihide"}], "nameIdentifiers": [{"nameIdentifier": "24589", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Sasaki, Hitoshi"}], "nameIdentifiers": [{"nameIdentifier": "24590", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2020-12-21"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "BPBul37_1274.pdf", "filesize": [{"value": "1.2 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 1200000.0, "url": {"label": "BPBul37_1274.pdf", "url": "https://nagasaki-u.repo.nii.ac.jp/record/5726/files/BPBul37_1274.pdf"}, "version_id": "a0f44e3b-0cb4-4185-a5a6-79ff27abd305"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "Gene delivery", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Small interfering RNA", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Ternary complex", "subitem_subject_scheme": "Other"}, {"subitem_subject": "γ-polyglutamic acid", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Novel siRNA Delivery System Using a Ternary Polymer Complex with Strong Silencing Effect and No Cytotoxicity", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Novel siRNA Delivery System Using a Ternary Polymer Complex with Strong Silencing Effect and No Cytotoxicity"}]}, "item_type_id": "2", "owner": "2", "path": ["9"], "permalink_uri": "http://hdl.handle.net/10069/34813", "pubdate": {"attribute_name": "公開日", "attribute_value": "2014-10-21"}, "publish_date": "2014-10-21", "publish_status": "0", "recid": "5726", "relation": {}, "relation_version_is_last": true, "title": ["Novel siRNA Delivery System Using a Ternary Polymer Complex with Strong Silencing Effect and No Cytotoxicity"], "weko_shared_id": -1}
Novel siRNA Delivery System Using a Ternary Polymer Complex with Strong Silencing Effect and No Cytotoxicity
http://hdl.handle.net/10069/34813
http://hdl.handle.net/10069/34813b7000542-198a-4eb1-adce-29914fe6b8f2
名前 / ファイル | ライセンス | アクション |
---|---|---|
BPBul37_1274.pdf (1.2 MB)
|
|
Item type | 学術雑誌論文 / Journal Article(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2014-10-21 | |||||
タイトル | ||||||
タイトル | Novel siRNA Delivery System Using a Ternary Polymer Complex with Strong Silencing Effect and No Cytotoxicity | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Gene delivery | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Small interfering RNA | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Ternary complex | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | γ-polyglutamic acid | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Kodama, Yukinobu
× Kodama, Yukinobu× Shiokawa, Yumi× Nakamura, Tadahiro× Kurosaki, Tomoaki× Aki, Keisei× Nakagawa, Hiroo× Muro, Takahiro× Kitahara, Takashi× Higuchi, Norihide× Sasaki, Hitoshi |
|||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | We developed a novel small interfering RNA (siRNA) delivery system using a ternary complex with polyethyleneimine (PEI) and γ-polyglutamic acid (γ-PGA), which showed silencing effect and no cytotoxicity. The binary complexes of siRNA with PEI were approximately 73-102 nm in particle size and 45-52 mV in a-potential. The silencing effect of siRNA/PEI complexes increased with an increase of PEI, and siRNA/PEI complexes with a charge ratio greater than 16 showed significant luciferase knockdown in a mouse colon carcinoma cell line regularly expressing luciferase (Colon26/Luc cells). However, strong cytotoxicity and blood agglutination were observed in the siRNA/Lipofectamine complex and siRNA/PEI16 complex. Recharging cationic complexes with an anionic compound was reported to be a promising method for overcoming these toxicities. We therefore prepared ternary complexes of siRNA with PEI (charge ratio 16) by the addition of γ-PGA to reduce cytotoxicity and deliver siRNA. As expected, the cytotoxicity of the ternary complexes decreased with an increase of γ-PGA content, which decreased the a-potential of the complexes. A strong silencing effect comparable to siRNA/Lipofectamine complex was discovered in ternary complexes including γ-PGA with an anionic surface charge. The high incorporation of ternary complexes into Colon26/Luc cells was confirmed with fluorescence microcopy. Having achieved knockdown of an exogenously transfected gene, the ability of the complex to mediate knockdown of an endogenous housekeeping gene, glyceraldehyde 3-phosphate dehydrogenase (GAPDH), was assessed in B16-F10 cells. The ternary complex (siRNA/PEI16/γ-PGA12 complex) exhibited a significant GAPDH knockdown effect. Thus, we developed a useful siRNA delivery system. | |||||
書誌情報 |
Biological and Pharmaceutical Bulletin 巻 37, 号 8, p. 1274-1281, 発行日 2014-08-01 |
|||||
出版者 | ||||||
出版者 | 日本薬学会 | |||||
出版者別言語 | ||||||
Pharmaceutical Society of Japan | ||||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 09186158 | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 13475215 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1248/bpb.b14-00058 | |||||
権利 | ||||||
権利情報 | c 2014 The Pharmaceutical Society of Japan | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Biological and Pharmaceutical Bulletin, 37(8), pp.1274-1281; 2014 |