WEKO3
アイテム
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Antitumor effect of nuclear factor-κB decoy transfer by mannose-modified bubble lipoplex into macrophages in mouse malignant ascites
http://hdl.handle.net/10069/34832
http://hdl.handle.net/10069/34832ac79f4ae-0fc5-4ad3-8796-b96600057840
名前 / ファイル | ライセンス | アクション |
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CanSci105_1049.pdf (1.3 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2014-10-28 | |||||
タイトル | ||||||
タイトル | Antitumor effect of nuclear factor-κB decoy transfer by mannose-modified bubble lipoplex into macrophages in mouse malignant ascites | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Drug targeting | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Ehrlich ascites carcinoma | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Nuclear factor-κB decoy | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Sonoporation | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Tumor-associated macrophages | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Kono, Yusuke
× Kono, Yusuke× Kawakami, Shigeru× Higuchi, Yuriko× Maruyama, Kazuo× Yamashita, Fumiyoshi× Hashida, Mitsuru |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Patients with malignant ascites (MAs) display several symptoms, such as dyspnea, nausea, pain, and abdominal tenderness, resulting in a significant reduction in their quality of life. Tumor-associated macrophages (TAMs) play a crucial role in MA progression. Because TAMs have a tumor-promoting M2 phenotype, conversion of the M2 phenotypic function of TAMs would be promising for MA treatment. Nuclear factor-κB (NF-κB) is a master regulator of macrophage polarization. Here, we developed targeted transfer of a NF-κB decoy into TAMs by ultrasound (US)-responsive, mannose-modified liposome/NF-κB decoy complexes (Man-PEG bubble lipoplexes) in a mouse peritoneal dissemination model of Ehrlich ascites carcinoma. In addition, we investigated the effects of NF-κB decoy transfection into TAMs on MA progression and mouse survival rates. Intraperitoneal injection of Man-PEG bubble lipoplexes and US exposure transferred the NF-κB decoy into TAMs effectively. When the NF-κB decoy was delivered into TAMs by this method in the mouse peritoneal dissemination model, mRNA expression of the Th2 cytokine interleukin (IL)-10 in TAMs was decreased significantly. In contrast, mRNA levels of Th1 cytokines (IL-12, tumor necrosis factor-α, and IL-6) were increased significantly. Moreover, the expression level of vascular endothelial growth factor in ascites was suppressed significantly, and peritoneal angiogenesis showed a reduction. Furthermore, NF-κB decoy transfer into TAMs significantly decreased the ascitic volume and number of Ehrlich ascites carcinoma cells in ascites, and prolonged mouse survival. In conclusion, we transferred a NF-κB decoy efficiently by Man-PEG bubble lipoplexes with US exposure into TAMs, which may be a novel approach for MA treatment. We demonstrated that the combinatorial use of mannose-modified bubble lipoplexes with ultrasound exposure achieved the efficient delivery of NF-κB decoy oligonucleotides into tumor-associated macrophages (TAM) in a mouse peritoneal dissemination model of Ehrlich ascites carcinoma. Using this method, NF-κB decoy transfer into TAM inhibited the malignant ascites progression significantly that may be the phenotypic conversion of TAM from M2 toward M1. | |||||
書誌情報 |
Cancer Science 巻 105, 号 8, p. 1049-1055, 発行日 2014-08 |
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出版者 | ||||||
出版者 | 日本癌学会 | |||||
出版者別言語 | ||||||
Japanese Cancer Association | ||||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 13479032 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1111/cas.12452 | |||||
権利 | ||||||
権利情報 | c 2014 The Authors. Cancer Science published by Wiley Publishing Asia Pty Ltd on behalf of Japanese Cancer Association. | |||||
権利 | ||||||
権利情報 | This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Cancer Science, 105(8), pp.1049-1055; 2014 |