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Reevaluation of bactericidal, cytotoxic, and macrophage-stimulating activities of commercially available Fucus vesiculosus fucoidan
http://hdl.handle.net/10069/34882
http://hdl.handle.net/10069/34882ae76e325-44ff-4cd1-8d20-d1d6b1de13c2
名前 / ファイル | ライセンス | アクション |
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ALGAE29_237.pdf (1.9 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2014-11-14 | |||||
タイトル | ||||||
タイトル | Reevaluation of bactericidal, cytotoxic, and macrophage-stimulating activities of commercially available Fucus vesiculosus fucoidan | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Antibacterial activity | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Contaminants | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Cytotoxic activity | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Fucoidan | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Fucus vesiculosus | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Nishiguchi, Tomoki
× Nishiguchi, Tomoki× Jiang, Zedong× Ueno, Mikinori× Takeshita, Satoshi× Cho, Kichul× Roh, Seong Woon× Kang, Kyong-Hwa× Yamaguchi, Kenichi× Kim, Daekyung× Oda, Tatsuya |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Polysaccharides prepared from marine algae sometimes contain contaminants such as polyphenols and endotoxins that may mislead their bona fide biological activities. In this study, we examined bioactive contaminants in commercially available fucoindan from Fucus vesiculosus, along with ascophyllan and fucoidan from Ascophyllum nodosum. F. vesiculosus fucoidan inhibited the growth of Vibrio alginolyticus in a concentration-dependent manner (0-1,000 μg mL?1). However, the antibacterial activity of the fucoidan significantly reduced after methanol-extraction, and the methanol-extract showed a potent antibacterial activity. The extract also showed cytotoxicity to RAW264.7 and U937 cells, and induced apoptotic nuclear morphological changes in U937 cells. These results suggest that the antibacterial activity of the fucoidan is partly due to the methanol-extractable contaminants that can also contribute to the cytotoxicity on RAW264.7 and U937 cells. On the other hand, the activities to induce secretion of nitric oxide and tumor necrosis factor-α from RAW264.7 cells were observed in the fucoidan even after methanol extraction, and the extract had no such activities. Our observations suggest that commercially available fucoidan should be purified prior to biochemical use. | |||||
書誌情報 |
ALGAE 巻 29, 号 3, p. 237-247, 発行日 2014-09 |
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出版者 | ||||||
出版者 | The Korean Society of Phycology | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 12262617 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.4490/algae.2014.29.3.237 | |||||
権利 | ||||||
権利情報 | c 2014 The Korean Society of Phycology | |||||
権利 | ||||||
権利情報 | This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | ALGAE, 29(3), pp.237-247; 2014 |