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Detection of Plasmodium knowlesi DNA in the urine and faeces of a Japanese macaque (Macaca fuscata) over the course of an experimentally induced infection
http://hdl.handle.net/10069/34900
http://hdl.handle.net/10069/34900a960da27-1cb3-4106-842b-849df579592e
名前 / ファイル | ライセンス | アクション |
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MalJou13_373.pdf (948.9 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2014-11-19 | |||||
タイトル | ||||||
タイトル | Detection of Plasmodium knowlesi DNA in the urine and faeces of a Japanese macaque (Macaca fuscata) over the course of an experimentally induced infection | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Chloroquine sulphate | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Faeces | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Japanese macaque | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Nested PCR | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Cytochrome b | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Plasmodium knowlesi | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Urine | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Zoonotic malaria | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Kawai, Satoru
× Kawai, Satoru× Sato, Megumi× Kato-Hayashi, Naoko× Kishi, Hisashi× Huffman, Michael A× Maeno, Yoshimasa× Culleton, Richard× Nakazawa, Shusuke |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Results: Urinary PkDNA was detected on day 2, but was not amplified using DNA templates extracted from the samples on day 4, day 5 and day 6. Subsequently, urinary PkDNA was detected from day 7 until day 11, and from day 20 until day 30. PkDNA in faeces was detected from day 7 until day 11, and from day 20 until day 37. Moreover, real-time quantitative PCR showed a remarkable increase in the amount of urinary PkDNA following anti-malarial treatment. This might have been due to the release of a large amount of PkDNA from the degraded parasites as a result of the anti-malarial treatment, leading to excretion of PkDNA in the urine. Methods. Urine and faeces were obtained from a Plasmodium knowlesi infected-Japanese macaque (Macaca fuscata) over the course of an experimentally induced infection. P. knowlesi DNA (PkDNA) extracted from urine and faeces were monitored by nested PCR targeting the P. knowlesi specific cytochrome b (cytb) gene. Background: Diagnostic techniques based on PCR for the detection of Plasmodium DNA can be highly sensitive and specific. The vast majority of these techniques rely, however, on the invasive sampling of blood from infected hosts. There is, currently, considerable interest in the possibility of using body fluids other than blood as sources of parasite DNA for PCR diagnosis. Conclusions: The cytb-PCR system using urine and faecal samples is of potential use in molecular epidemiological surveys of malaria. In particular, monkey faecal samples could be useful for the detection of zoonotic primate malaria in its natural hosts. |
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書誌情報 |
Malaria Journal 巻 13, 号 1, p. 373, 発行日 2014-09-19 |
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出版者 | ||||||
出版者 | BioMed Central | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 14752875 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1186/1475-2875-13-373 | |||||
権利 | ||||||
権利情報 | c 2014 Kawai et al.; licensee BioMed Central Ltd. | |||||
権利 | ||||||
権利情報 | This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver ( http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Malaria Journal, 13(1), 373; 2014 |