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Production of domoic acid by laboratory culture of the red alga Chondria armata
http://hdl.handle.net/10069/34904
http://hdl.handle.net/10069/349049b833d65-fdc6-49e7-b95d-5852f785e190
名前 / ファイル | ライセンス | アクション |
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Toxicon92_1.pdf (235.6 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2014-11-20 | |||||
タイトル | ||||||
タイトル | Production of domoic acid by laboratory culture of the red alga Chondria armata | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Amnesic shellfish poisoning (ASP) | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Chondria armata | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Domoic acid (DA) | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Laboratory culture | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Modified PES medium | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Red alga | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Jiang, Shanshan
× Jiang, Shanshan× Kuwano, Kazuyoshi× Ishikawa, Nozomi× Yano, Michiko× Takatani, Tomohiro× Arakawa, Osamu |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | To clarify the production mechanisms and biologic functions of domoic acid (DA) by the red alga Chondria armata, we established a laboratory culture of C. armata. The alga grew better in modified PES medium (mPES) without trace metals or manganese than in unmodified mPES (seawater + nitrate, phosphate, iron, trace metals, vitamins, and 2-[4-(2-hydroxyethyl)-1-piperazinyl]-ethanesulfonic acid), suggesting that C. armata is especially hypersensitive to the toxicity of excessive manganese. C. armata cultured in N・P・Fe medium (seawater + nitrate, phosphate, and iron) grew best (mean growth rate 828.4%) at a relative nutrient concentration of 50%. Liquid chromatography-mass spectrometry analysis of the algal extracts revealed that the DA content of the cultured explants (2273-3308 ppm) was 4-5 fold higher than that of wild specimens. The extract of pooled explants (60 g) was purified by activated charcoal treatment and several types of column chromatography to afford ca. 10 mg DA. The 1H-nuclear magnetic resonance spectrum of the preparation was indistinguishable from the previously reported spectrum of DA, indicating that C. armata itself has an ability to produce DA. | |||||
書誌情報 |
Toxicon 巻 92, p. 1-5, 発行日 2014-12-15 |
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出版者 | ||||||
出版者 | Elsevier Limited | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 00410101 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1016/j.toxicon.2014.09.003 | |||||
権利 | ||||||
権利情報 | c 2014 Elsevier Ltd. | |||||
権利 | ||||||
権利情報 | NOTICE: this is the author’s version of a work that was accepted for publication in Toxicon. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Toxicon, 92, (2014) | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Toxicon, 92, pp.1-5; 2014 |