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Origin of a novel protein-coding gene family with similar signal sequence in Schistosoma japonicum
http://hdl.handle.net/10069/34913
http://hdl.handle.net/10069/34913076608fa-4e0b-40fc-988a-7cfceaca1e24
名前 / ファイル | ライセンス | アクション |
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BMCGen13_26.pdf (2.2 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2014-11-26 | |||||
タイトル | ||||||
タイトル | Origin of a novel protein-coding gene family with similar signal sequence in Schistosoma japonicum | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Signal sequence trap | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Schistosoma japonicum | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Repetitive elements | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Gene duplication | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Secreted proteins | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Non-allelic homologous recombination | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Mbanefo, Evaristus
× Mbanefo, Evaristus× Chuanxin, Yu× Kikuchi, Mihoko× Shuaibu, Mohammed× Boamah, Daniel× Kirinoki, Masashi× Hayashi, Naoko× Chigusa, Yuichi× Osada, Yoshio× Hamano, Shinjiro× Hirayama, Kenji |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Background Evolution of novel protein-coding genes is the bedrock of adaptive evolution. Recently, we identified six protein-coding genes with similar signal sequence from Schistosoma japonicum egg stage mRNA using signal sequence trap (SST). To find the mechanism underlying the origination of these genes with similar core promoter regions and signal sequence, we adopted an integrated approach utilizing whole genome, transcriptome and proteome database BLAST queries, other bioinformatics tools, and molecular analyses. Results Our data, in combination with database analyses showed evidences of expression of these genes both at the mRNA and protein levels exclusively in all developmental stages of S. japonicum. The signal sequence motif was identified in 27 distinct S. japonicum UniGene entries with multiple mRNA transcripts, and in 34 genome contigs distributed within 18 scaffolds with evidence of genome-wide dispersion. No homolog of these genes or similar domain was found in deposited data from any other organism. We observed preponderance of flanking repetitive elements (REs), albeit partial copies, especially of the RTE-like and Perere class at either side of the duplication source locus. The role of REs as major mediators of DNA-level recombination leading to dispersive duplication is discussed with evidence from our analyses. We also identified a stepwise pathway towards functional selection in evolving genes by alternative splicing. Equally, the possible transcription models of some protein-coding representatives of the duplicons are presented with evidence of expression in vitro. Conclusion Our findings contribute to the accumulating evidence of the role of REs in the generation of evolutionary novelties in organisms' genomes. |
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書誌情報 |
BMC Genomics 巻 13, p. 260, 発行日 2012-06-20 |
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出版者 | ||||||
出版者 | BioMed Central Ltd. | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 14712164 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1186/1471-2164-13-260 | |||||
権利 | ||||||
権利情報 | c 2012 Mbanefo et al.; licensee BioMed Central Ltd. | |||||
権利 | ||||||
権利情報 | This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | BMC Genomics, 13, 260; 2012 |