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Analytical method for lipoperoxidation relevant reactive aldehydes in human sera by high-performance liquid chromatography?fluorescence detection
http://hdl.handle.net/10069/34987
http://hdl.handle.net/10069/3498756c6a587-f776-4133-8c1a-3ffc67ba19e0
名前 / ファイル | ライセンス | アクション |
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AnaBio464_36.pdf (592.8 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2015-01-08 | |||||
タイトル | ||||||
タイトル | Analytical method for lipoperoxidation relevant reactive aldehydes in human sera by high-performance liquid chromatography?fluorescence detection | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 2,2′-Furil | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Aldehydes | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Diabetes | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | HPLC-fluorescence detection | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Lipoperoxidation | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Rheumatoid arthritis | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
El-Maghrabey, Mahmoud H.
× El-Maghrabey, Mahmoud H.× Kishikawa, Naoya× Ohyama, Kaname× Kuroda, Naotaka |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | A validated, simple and sensitive HPLC method was developed for the simultaneous determination of lipoperoxidation relevant reactive aldehydes: glyoxal (GO), acrolein (ACR), malondialdehyde (MDA), and 4-hydroxy-2-nonenal (HNE) in human serum. The studied aldehydes were reacted with 2,2′-furil to form fluorescent difurylimidazole derivatives that were separated on a C 18 column using gradient elution and fluorescence detection at excitation and emission wavelengths of 250 and 355 nm, respectively. The method showed good linearity over the concentration ranges of 0.100-5.00, 0.200-10.0, 0.200-40.0, and 0.400-10.0 nmol/mL for GO, ACR, HNE, and MDA, respectively, with detection limits ranging from 0.030 to 0.11 nmol/mL. The percentage RSD of intraday and interday precision did not exceed 5.0 and 6.2%, respectively, and the accuracy (%found) ranged from 95.5 to 103%. The proposed method was applied for monitoring the four aldehydes in sera of healthy, diabetic, and rheumatic human subjects with simple pretreatment steps and without interference from endogenous components. By virtue of its high sensitivity and accuracy, our method enabled detection of differences between analytes concentrations in sera of human subjects under different clinical conditions. | |||||
書誌情報 |
Analytical Biochemistry 巻 464, p. 36-42, 発行日 2014-11-01 |
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出版者 | ||||||
出版者 | Academic Press Inc. | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 00032697 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1016/j.ab.2014.07.002 | |||||
権利 | ||||||
権利情報 | c 2014 Elsevier Inc. | |||||
権利 | ||||||
権利情報 | NOTICE: this is the author’s version of a work that was accepted for publication in Analytical Biochemistry. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Analytical Biochemistry, 464, (2014) | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Analytical Biochemistry, 464, pp.36-42; 2014 |