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Plasmodium knowlesi thioredoxin peroxidase 1 binds to nucleic acids and has RNA chaperone activity
http://hdl.handle.net/10069/35012
http://hdl.handle.net/10069/3501274bf278f-7b8e-4caa-a64b-08645ec199f6
名前 / ファイル | ライセンス | アクション |
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ParRes113_3957.pdf (1.3 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2015-12-01 | |||||
タイトル | ||||||
タイトル | Plasmodium knowlesi thioredoxin peroxidase 1 binds to nucleic acids and has RNA chaperone activity | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Nucleic acid binding | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Plasmodium knowlesi | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | RNA chaperone | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Thioredoxin peroxidase 1 | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Hakimi, Hassan
× Hakimi, Hassan× Suganuma, Keisuke× Usui, Miho× Masuda-Suganuma, Hirono× Angeles, Jose Ma. M.× Asada, Masahito× Kawai, Satoru× Inoue, Noboru× Kawazu, Shin-ichiro |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Malaria parasites are under oxidative attack throughout their life cycle in human body and mosquito vector. Therefore, Plasmodium antioxidant defenses are crucial for its survival and being considered as interesting target for antimalarial drug design. Plasmodium knowlesi has emerged recently from its simian host to human in Southeast Asia and has been recognized as the fifth Plasmodium species that can cause human malaria. In this study, we cloned and characterized thioredoxin peroxidase 1 from P. knowlesi (PkTPx-1). PkTPx-1 gene was cloned, and recombinant protein was produced by heterologous overexpression in Escherichia coli. The recombinant protein was used for evaluation of enzymatic activity and polyclonal antibody production. Using the recombinant PkTPx-1 protein, its antioxidant activity was confirmed in a mixed-function oxidation assay where PkTPx-1 prevented nicking of DNA by hydroxyl radicals. PkTPx-1 was able to bind to double-strand DNA and RNA and had RNA chaperone activity in a nucleic acid melting assay indicating new function of PkTPx-1 other than antioxidant activity. Using specific polyclonal antibodies, it was indicated that PkTPx-1 is expressed in the cytoplasm of the parasite. Altogether, these results suggest that PkTPx-1 not only protects the parasite from the adverse effects of reactive oxygen species but also has RNA chaperone activity. | |||||
書誌情報 |
Parasitology Research 巻 113, 号 11, p. 3957-3962, 発行日 2014-11 |
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出版者 | ||||||
出版者 | Springer Verlag | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 09320113 | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 14321955 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1007/s00436-014-4060-0 | |||||
権利 | ||||||
権利情報 | c Springer-Verlag Berlin Heidelberg 2014 | |||||
権利 | ||||||
権利情報 | The final publication is available at link.springer.com | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Parasitology Research, 113(11), pp.3957-3962; 2014 |