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Localization of three forms of gonadotropin-releasing hormone in the brain and pituitary of the self-fertilizing fish, Kryptolebias marmoratus
http://hdl.handle.net/10069/39770
http://hdl.handle.net/10069/39770ea86d338-9476-49be-aa91-baad714e85c1
名前 / ファイル | ライセンス | アクション |
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FPB45_753.pdf (2.6 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2020-04-02 | |||||
タイトル | ||||||
タイトル | Localization of three forms of gonadotropin-releasing hormone in the brain and pituitary of the self-fertilizing fish, Kryptolebias marmoratus | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | GnRH | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | GTH | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Immunohistochemistry | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | In situ hybridization | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Self-fertilizing fish | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Brain | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Amano, Masafumi
× Amano, Masafumi× Amiya, Noriko× Okubo, Kataaki× Yamashita, Junpei× Kuriu, Ayae× Yasuta, Ayano× Yamamoto, Naoyuki× Sakakura, Yoshitaka |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | The localization of gonadotropin-releasing hormone (GnRH) in the brain and pituitary of the self-fertilizing mangrove killifish Kryptolebias marmoratus was examined by immunohistochemistry and in situ hybridization to understand its neuroendocrine system. The genome assembly of K. marmoratus did not have any sequence encoding GnRH1, but sequences encoding GnRH2 (chicken GnRH-II) and GnRH3 (salmon GnRH) were found. Therefore, GnRH1 was identified by in silico cloning. The deduced amino acid sequence of the K. marmoratus GnRH1 (mature peptide) was identical to that of the medaka GnRH. GnRH1 neurons were detected in the ventral part of the preoptic nucleus by immunohistochemistry and in situ hybridization, and GnRH1-immunoreactive (ir) fibers were observed throughout the brain. GnRH1-ir fibers were in close contact with luteinizing hormone (LH)-ir cells in the pituitary using double immunohistochemistry. GnRH2 neurons were detected in the midbrain tegmentum by immunohistochemistry and in situ hybridization. Although GnRH2-ir fibers were observed throughout the brain, they were not detected in the pituitary. GnRH3 neurons were detected in the lateral part of the ventral telencephalic area by both methods. GnRH3-ir fibers were observed throughout the brain, and a few GnRH3-ir fibers were in close contact with LH-ir cells in the pituitary. These results indicate that GnRH1 and possibly GnRH3 are responsible for gonadal maturation through LH secretion and that all three forms of GnRH function as neurotransmitters or neuromodulators in the brain of K. marmoratus. | |||||
書誌情報 |
Fish Physiology and Biochemistry 巻 45, 号 2, p. 753-771, 発行日 2019-01-07 |
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出版者 | ||||||
出版者 | Springer Nature | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 09201742 | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 15735168 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1007/s10695-018-0601-2 | |||||
権利 | ||||||
権利情報 | c Springer Nature B.V. 2019 This is a post-peer-review, pre-copyedit version of an article published in Fish Physiology and Biochemistry. The final authenticated version is available online at: http://dx.doi.org/10.1007/s10695-018-0601-2. | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Fish Physiology and Biochemistry, 45(2), pp.753-771; 2019 |