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Characterization of substrate specificity and novel autoprocessing mechanism of dipeptidase A from Prevotella intermedia
http://hdl.handle.net/10069/39777
http://hdl.handle.net/10069/397772252214f-ef03-4ff7-bc46-e0e5cc06479c
名前 / ファイル | ライセンス | アクション |
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ISYK1215_Sarwar.pdf (3.9 MB)
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Item type | 学位論文 / Thesis or Dissertation(1) | |||||
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公開日 | 2020-04-07 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Characterization of substrate specificity and novel autoprocessing mechanism of dipeptidase A from Prevotella intermedia | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | dipeptidase A | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | cysteine peptidase | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | autoprocessing | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Prevotella intermedia | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | substrate specificity | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | periodontal disease | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||
資源タイプ | doctoral thesis | |||||
アクセス権 | ||||||
アクセス権 | open access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
著者 |
SARWAR, MOHAMMAD TANVIR
× SARWAR, MOHAMMAD TANVIR |
|||||
著者別名 | ||||||
姓名 | サルワル, モハマド タンビール | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Prevotella intermedia, a gram-negative anaerobic rod, is frequently observed in subgingival polymicrobial biofilm from adults with chronic periodontitis. Peptidases in periodontopathic bacteria are considered to function as etiological reagents. Pre. intermedia OMA14 cells abundantly express an unidentified cysteine peptidase specific for Arg-4-methycoumaryl-7- amide (MCA). BAU17746 (locus tag, PIOMA14_I_1238) and BAU18827 (locus tag, PIOMA14_II_0322) emerged as candidates of this peptidase from the substrate specificity and sequence similarity with C69-family Streptococcus gordonii Arg-aminopeptidase. The recombinant form of the former solely exhibited hydrolyzing activity toward Arg-MCA, and BAU17746 possesses a 26.6% amino acid identity with the C69-family Lactobacillus helveticus dipeptidase A. It was found that BAU17746 as well as L. helveticus dipeptidase A was a P1-position Arg-specific dipeptidase A, although the L. helveticus entity, a representative of the C69 family, had been reported to be specific for Leu and Phe. The fulllength form of BAU17746 was intramolecularly processed to a mature form carrying the N-terminus of Cys15. In conclusion, the marked Arg-MCA-hydrolyzing activity in Pre. intermedia was mediated by BAU17746 belonging to the C69-family dipeptidase A, in which the mature form carries an essential cysteine at the N-terminus. | |||||
内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 長崎大学学位論文 学位記番号:博(医歯薬)甲第1215号 学位授与年月日:令和2年3月19日 | |||||
内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Author: Mohammad Tanvir Sarwar, Yuko Ohara-Nemoto, Takeshi Kobayakawa, Mariko Naito and Takayuki K. Nemoto | |||||
内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Citation: Biological Chemistry, 401(5), pp. 629-642 ; 2020 | |||||
書誌情報 |
Biological Chemistry 巻 401, 号 5, p. 629-642, 発行日 2020-03-19 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 14316730 | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 14374315 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1515/hsz-2019-0387 | |||||
権利 | ||||||
権利情報 | c 2020 by Walter de Gruyter Berlin/Boston. The final publication is available at www.degruyter.com | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
その他のタイトル | ||||||
その他のタイトル | Prevotella intermedia ジペプチダーゼAの基質特異性の決定と新規オートプロセシング機構 | |||||
出版者 | ||||||
出版者 | Walter de Gruyter | |||||
関係URI | ||||||
識別子タイプ | HDL | |||||
関連識別子 | http://hdl.handle.net/10069/39913 | |||||
学位名 | ||||||
学位名 | 博士(歯学) | |||||
学位授与機関 | ||||||
学位授与機関識別子Scheme | kakenhi | |||||
学位授与機関識別子 | 17301 | |||||
学位授与機関名 | Nagasaki University (長崎大学) | |||||
学位授与年月日 | ||||||
学位授与年月日 | 2020-03-19 | |||||
学位授与番号 | ||||||
学位授与番号 | 甲医歯薬第1215号 | |||||
学位の種類 | ||||||
課程博士 | ||||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Nagasaki University (長崎大学), 博士(歯学) (2020-03-19) |