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A novel lophine-based fluorescence probe and its binding to human serum albumin
http://hdl.handle.net/10069/33128
http://hdl.handle.net/10069/331282b5e0d82-a6a7-4ccf-b2a3-88b1d5e31b8e
名前 / ファイル | ライセンス | アクション |
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ACA780_1.pdf (836.0 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2013-08-05 | |||||
タイトル | ||||||
タイトル | A novel lophine-based fluorescence probe and its binding to human serum albumin | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 4-[4-(4-Dimethylaminophenyl)-5-phenyl-1H-imidazol-2-yl]benzoic acid methyl ester | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Binding constant | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Binding site | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Human serum albumin | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Kishikawa, Naoya
× Kishikawa, Naoya× Ohyama, Kaname× Saiki, Akane× Matsuo, Aya× Ali, Marwa Fathy Bakr× Wada, Mitsuhiro× Nakashima, Kenichiro× Kuroda, Naotaka |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | The binding of a lophine-based fluorescence probe, 4-[4-(4-dimethylaminophenyl)-5-phenyl-1H-imidazol-2-yl]benzoic acid methyl ester (DAPIM) with human serum albumin (HSA) was investigated by fluorescence spectroscopy under physiological conditions. While DAPIM shows extreme low fluorescence in aqueous solution, DAPIM binding with HSA emits strong fluorescence at 510nm. The binding constant and binding number determined by Scatchard plot was 3.65×106M-1 and 1.07, respectively. Competitive binding between DAPIM and other ligands such as warfarin, valproic acid, diazepam and oleic acid, were also studied fluorometrically. The results indicated that the primary binding site of DAPIM to HSA is site II at subdomain IIIA. DAPIM can be a useful fluorescence probe for the characterization of drug-binding sites. In addition to the interaction study, because the fluorescence intensity of DAPIM increased in proportion to HSA concentration, its potential in HSA assay for serum sample was also evaluated. | |||||
書誌情報 |
Analytica Chimica Acta 巻 780, p. 1-6, 発行日 2013-05-30 |
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出版者 | ||||||
出版者 | Elsevier | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 00032670 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1016/j.aca.2013.04.003 | |||||
権利 | ||||||
権利情報 | © 2013 Published by Elsevier B.V. | |||||
権利 | ||||||
権利情報 | NOTICE: this is the author’s version of a work that was accepted for publication in Analytica Chimica Acta. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Analytica Chimica Acta, 780(2013) | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Analytica Chimica Acta, 780, pp.1-6; 2013 |