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Establishment of Novel High-Standard Chemiluminescent Assay for NTPase in Two Protozoans and Its High-Throughput Screening
http://hdl.handle.net/10069/39799
http://hdl.handle.net/10069/397990aa19635-caa1-47d6-9c79-b56d0053c055
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
MarDru18_161.pdf (1.3 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2020-04-10 | |||||
| タイトル | ||||||
| タイトル | Establishment of Novel High-Standard Chemiluminescent Assay for NTPase in Two Protozoans and Its High-Throughput Screening | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Toxoplasma gondii | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Neospora caninum | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | NTPase | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | HTS | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | synthetic compound library | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | marine bacteria extracts | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 著者 |
Harada, Masamitsu
× Harada, Masamitsu× Nagai, Jun× Kurata, Riho× Shimizu, Kenji× Cui, Xiaofeng× Isagawa, Takayuki× Semba, Hiroaki× Ishihara, Jun× Yoshida, Yasuhiro× Takeda, Norihiko× Maemura, Koji× Yonezawa, Tomo |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Toxoplasma gondii is a major protozoan parasite and infects human and many other warm-blooded animals. The infection leads to Toxoplasmosis, a serious issue in AIDS patients, organ transplant recipients and pregnant women. Neospora caninum, another type of protozoa, is closely related to Toxoplasma gondii. Infections of the protozoa in animals also causes serious diseases such as Encephalomyelitis and Myositis-Polyradiculitis in dogs or abortion in cows. Both Toxoplasma gondii and Neospora caninum have similar nucleoside triphosphate hydrolases (NTPase), NcNTPase and TgNTPase-I in Neospora caninum and Toxoplasma gondii, respectively. These possibly play important roles in propagation and survival. Thus, we targeted the enzymes for drug discovery and tried to establish a novel high-standard assay by a combination of original biochemical enzyme assay and fluorescent assay to determine ADP content. We then validated whether or not it can be applied to high-throughput screening (HTS). Then, it fulfilled criterion to carry out HTS in both of the enzymes. In order to identify small molecules having inhibitory effects on the protozoan enzyme, we also performed HTS using two synthetic compound libraries and an extract library derived from marine bacteria and then, identified 19 compounds and 6 extracts. Nagasaki University collected many extracts from over 18,000 marine bacteria found in local Omura bay, and continues to compile an extensive collection of synthetic compounds from numerous drug libraries established by Japanese chemists. | |||||
| 書誌情報 |
Marine Drugs 巻 18, 号 3, p. 161, 発行日 2020-03-13 |
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| 出版者 | ||||||
| 出版者 | MDPI | |||||
| EISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 16603397 | |||||
| DOI | ||||||
| 関連タイプ | isIdenticalTo | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.3390/md18030161 | |||||
| 権利 | ||||||
| 権利情報 | c 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| 引用 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Marine Drugs, 18(3), art.no.161; 2020 | |||||
