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Biochemical Features of a Catalytic Antibody Light Chain, 22F6, Prepared from Human Lymphocytes
http://hdl.handle.net/10069/33594
http://hdl.handle.net/10069/335949bf6bc5e-3f68-4141-b981-668aa13fbb24
名前 / ファイル | ライセンス | アクション |
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JBC288_19558.pdf (1.5 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2013-09-06 | |||||
タイトル | ||||||
タイトル | Biochemical Features of a Catalytic Antibody Light Chain, 22F6, Prepared from Human Lymphocytes | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Hifumi, Emi
× Hifumi, Emi× Fujimoto, Naoko× Arakawa, Mitsue× Saito, Eri× Matsumoto, Shingo× Kobayashi, Nobuyuki× Uda, Taizo |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Human antibody light chains belonging to subgroup II of germ line genes were amplified by a seminested PCR technique using B-lymphocytes taken from a human adult infected with influenza virus. Each gene of the human light chains was transferred into the Escherichia coli system. The recovered light chain was highly purified using a two-step purification system. Light chain 22F6 showed interesting catalytic features. The light chain cleaved a peptide bond of synthetic peptidyl-4-methylcoumaryl- 7-amide (MCA) substrates, such as QAR-MCA and EAR-MCA, indicating amidase activity. It also hydrolyzed a phosphodiester bond of both DNA and RNA. From the analysis of amino acid sequences and molecular modeling, the 22F6 light chain possesses two kinds of active sites as amidase and nuclease in close distances. The 22F6 catalytic light chain could suppress the infection of influenza virus type A (H1N1) of Madin-Darby canine kidney cells in an in vitro assay. In addition, the catalytic light chain clearly inhibited the infection of the influenza virus of BALB/c mice via nasal administration in an in vivo assay. In the experiment, the titer in the serum of the mice coinfected with the 22F6 light chain and H1N1 virus became considerably lowered compared with that of 22F6-non-coinfected mice. Note that the catalytic light chain was prepared from human peripheral lymphocyte and plays an important role in preventing infection by influenza virus. Considering the fact that the human light chain did not show any acute toxicity for mice, our procedure developed in this study must be unique and noteworthy for developing new drugs. | |||||
書誌情報 |
Journal of Biological Chemistry 巻 288, 号 27, p. 19558-19568, 発行日 2013-07-05 |
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出版者 | ||||||
出版者 | American Society for Biochemistry and Molecular Biology Inc. | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 00219258 | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1083351X | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1074/jbc.M113.454579 | |||||
権利 | ||||||
権利情報 | © 2013 by The American Society for Biochemistry and Molecular Biology, Inc. | |||||
権利 | ||||||
権利情報 | This is author's choice open-access article distributed under the terms of the Creative Commons Attribution License. | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Journal of Biological Chemistry, 288(27), pp.19558-19568; 2013 |