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Measurement of protease activity of exfoliative toxin A using synthetic peptidyl substrates and correlation between in vivo and in vitro activities
http://hdl.handle.net/10069/33821
http://hdl.handle.net/10069/33821cf5a4412-3383-4b55-8085-87ef6c54081d
名前 / ファイル | ライセンス | アクション |
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ActMed58_41.pdf (4.0 MB)
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2013-09-25 | |||||
タイトル | ||||||
タイトル | Measurement of protease activity of exfoliative toxin A using synthetic peptidyl substrates and correlation between in vivo and in vitro activities | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Staphylococcus aureus | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | exfoliative toxin | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | desmoglein | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | GluV8 | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
著者 |
Tachi, Mizuki T.
× Tachi, Mizuki T.× Ohara-Nemoto, Yuko× Baba, Tomomi T.× Kobayakawa, Takeshi× Fujita, Shuichi× Ikeda, Tohru× Ayuse, Takao× Oi, Kumiko× Nemoto, Takayuki K. |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Exfoliative toxin A (ETA) produced by Staphylococcus aureus causes bullous impetigo and staphylococcal scalded skin syndrome. The exfoliative activity of ETA is ascribed to its highly restricted degradation between Glu381-Gly382 of desmoglein 1, a component protein of desmosomes. Since the peptidase activity of ETA has been yet to be demonstrated other than desmoglein 1, the entity as a peptidase and its molecular mechanism remain to be elucidated. In the present study, we determined the peptidase activity using recombinant ETA molecules and synthetic fluorescent peptidyl substrates, while the exfoliative activity was examined by a neonatal mouse model. Although peptidase activity was trivial as compared with the S. aureus glutamyl endopeptidase GluV8, pro-ETA starting from Phe24 (Phe24-ETA) and the mature form from Glu39 (Glu39-ETA) exhibited the activities toward LLE-, AE-, and LE-MCA, but not toward LLQ-, LD- or AAA-MCA, indicating a Glu-specific endopeptidase activity. This activity was statistically higher in Glu39-ETA than Phe24-ETA and was inhibited by the serine protease inhibitor Pefabloc. Deletion of the α1 region at positions 42-56 as well as substitution of active Ser233 to Ala abrogated the peptidase activity. In accord with these results, intraepidermal blister formation and epidermolysis were induced more exclusively by Glu39-ETA than Phe24-ETA. ETAs without proteolytic activity as well as GluV8 did not cause an exfoliative reaction. These results suggest that the highly restricted Glu-specific endopeptidase activity of ETA is involved in exfoliative activity and that the α1 region is required for these functions. | |||||
書誌情報 |
Acta medica Nagasakiensia 巻 58, 号 2, p. 41-48, 発行日 2013-08 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 00016055 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA00508430 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
出版者 | ||||||
出版者 | Nagasaki University School of Medicine | |||||
出版者別言語 | ||||||
長崎大学医学部 | ||||||
関係URI | ||||||
関連名称 | http://hdl.handle.net/10069/34125 | |||||
sortkey | ||||||
2 | ||||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Acta medica Nagasakiensia, 58(2), pp.41-48; 2013 |