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Efficacy of AiiM, an N-Acylhomoserine Lactonase, against Pseudomonas aeruginosa in a Mouse Model of Acute Pneumonia
http://hdl.handle.net/10069/34031
http://hdl.handle.net/10069/34031497d39db-1563-4a5e-b25e-ec94350bdbfe
名前 / ファイル | ライセンス | アクション |
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AAC57_3653.pdf (1.9 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2014-03-01 | |||||
タイトル | ||||||
タイトル | Efficacy of AiiM, an N-Acylhomoserine Lactonase, against Pseudomonas aeruginosa in a Mouse Model of Acute Pneumonia | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | AiiM | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | mice | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | pneumonia | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Pseudomonas aeruginosa | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | quorum sensing | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Migiyama, Yohei
× Migiyama, Yohei× Kaneko, Yukihiro× Yanagihara, Katsunori× Morohoshi, Tomohiro× Morinaga, Yoshitomo× Nakamura, Shigeki× Miyazaki, Taiga× Hasegawa, Hiroo× Izumikawa, Koichi× Kakeya, Hiroshi× Kohrogi, Hirotsugu× Kohno, Shigeru |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Quorum sensing (QS) in Pseudomonas aeruginosa regulates the production of many virulence factors and plays an important role in the pathogenesis of P. aeruginosa infection. N-acyl homoserine lactones (AHL) are major QS signal molecules. Recently, a novel AHL-lactonase enzyme, AiiM, has been identified. The aim of this study was to evaluate the effect of AiiM on the virulence of P. aeruginosa in a mouse model of acute pneumonia. We developed a P. aeruginosa PAO1 strain harboring an AiiM-expressing plasmid. The production of several virulence factors by the AiiM-expressing strain was examined. Mice were intratracheally infected with an AiiM-expressing PAO1 strain. Lung histopathology, bacterial burden, and bronchoalveolar lavage (BAL) fluid were assessed at 24 h postinfection. AiiM expression in PAO1 reduced production of AHL-mediated virulence factors and attenuated cytotoxicity against human lung epithelial cells. In a mouse model of acute pneumonia, AiiM expression reduced lung injury and greatly improved the survival rates. The levels of proinflammatory cytokines and myeloperoxidase activity in BAL fluid were significantly lower in mice infected with AiiM-expressing PAO1. Thus, AiiM can strongly attenuate P. aeruginosa virulence in a mammalian model and is a potential candidate for use as a therapeutic agent against P. aeruginosa infection. | |||||
書誌情報 |
Antimicrobial Agents and Chemotherapy 巻 57, 号 8, p. 3653-3658, 発行日 2013-08 |
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出版者 | ||||||
出版者 | American Society for Microbiology | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 00664804 | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 10986596 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1128/AAC.00456-13 | |||||
権利 | ||||||
権利情報 | © 2013, American Society for Microbiology. All Rights Reserved. | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Antimicrobial Agents and Chemotherapy, 57(8), pp.3653-3658; 2013 |