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Ternary Complex of Plasmid DNA with Protamine and γ-Polyglutamic Acid for Biocompatible Gene Delivery System
http://hdl.handle.net/10069/34048
http://hdl.handle.net/10069/34048ff84943a-9332-4f18-bd8d-592fc12ec344
名前 / ファイル | ライセンス | アクション |
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BPBul36_1794.pdf (945.5 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2014-01-09 | |||||
タイトル | ||||||
タイトル | Ternary Complex of Plasmid DNA with Protamine and γ-Polyglutamic Acid for Biocompatible Gene Delivery System | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | γ-polyglutamic acid | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Gene delivery | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Nanoparticle | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Non-viral vector | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Protamine | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Kanda, Kosuke
× Kanda, Kosuke× Kodama, Yukinobu× Kurosaki, Tomoaki× Imamura, Masanobu× Nakagawa, Hiroo× Muro, Takahiro× Higuchi, Norihide× Nakamura, Tadahiro× Kitahara, Takashi× Honda, Masayuki× Sasaki, Hitoshi |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | The purpose of the present study was to investigate the usefulness of the ternary complex with protamine and γ-polyglutamic acid (γ-PGA), which are biodegradable materials for foods and medical products, as a safe gene delivery vector. We formed cationic binary complexes (plasmid DNA (pDNA)/protamine complexes) with high transfection efficiency. The binary complex showed slight toxicity probably related to its total cationic charge. Then, we formed ternary complexes (pDNA/protamine/γ-PGA complexes) by addition of anionic polymer, γ-PGA, and they showed no cytotoxicity. The transfection efficiency of the pDNA/protamine/γ-PGA complexes was as high as that of the pDNA/protamine complexes, although their zeta potentials were different. Inhibition study of the gene expressions in B16-F10 cells suggested that pDNA/protamine complexes were taken up by caveolae-mediated endocytosis and macropinocytosis. On the other hand, pDNA/protamine/ γ-PGA complexes were taken up by clathrin-mediated endocytosis and macropinocytosis. Thus, we succeeded in developing the ternary complex as a safe gene delivery vector with biocompatible materials. | |||||
書誌情報 |
Biological and Pharmaceutical Bulletin 巻 36, 号 11, p. 1794-1799, 発行日 2013-11-01 |
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出版者 | ||||||
出版者 | 日本薬学会 | |||||
出版者別言語 | ||||||
Pharmaceutical Society of Japan | ||||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 09186158 | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 13475215 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1248/bpb.b13-00479 | |||||
権利 | ||||||
権利情報 | © 2013 The Pharmaceutical Society of Japan. | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Biological and Pharmaceutical Bulletin, 36(11), pp.1794-1799; 2013 |