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Early gene and protein expression associated with osteoblast differentiation in response to fish collagen peptides powder
http://hdl.handle.net/10069/32139
http://hdl.handle.net/10069/3213944c46264-0561-40f6-88cb-3172d4db69c7
名前 / ファイル | ライセンス | アクション |
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DMJ32_233.pdf (1.9 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2013-06-05 | |||||
タイトル | ||||||
タイトル | Early gene and protein expression associated with osteoblast differentiation in response to fish collagen peptides powder | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Alkaline phosphatase activity | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Fish collagen peptide | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Osteoblast differentiation | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Real-time PCR | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Western blot analysis | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Yamada, Shizuka
× Yamada, Shizuka× Yoshizawa, Yuu× Kawakubo, Atsushi× Ikeda, Takeshi× Yanagiguchi, Kajiro× Hayashi, Yoshihiko |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | This study was designed to investigate the biological effects of fish collagen peptide (FCP) on human osteoblasts. Human osteoblasts were treated with 0.1% FCP, which was the optimal concentration confirmed by the increase in alkaline phosphatase activity. After one, three, five and seven days of culture, the number of FCP-treated cells increased significantly compared with untreated cells. In a real-time PCR analysis, the expression of osteocalcin, osteopontin, BMP-2 and integrin β3 mRNAs in FCP-treated cells showed increases compared with untreated cells after three days of culture. After seven days of culture, the expression levels of osteopontin and integrin β3 were still higher in the FCP-treated cells than in untreated cells. The production of osteocalcin, osteopontin and integrin β3 proteins in FCP-treated cells also showed increases after seven days of culture. Furthermore, FCP accelerated matrix mineralization in the cultures. The present study indicates the potential utility of FCP as a biomaterial. | |||||
書誌情報 |
Dental Materials Journal 巻 32, 号 2, p. 233-240, 発行日 2013-03-29 |
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出版者 | ||||||
出版者 | 日本歯科理工学会 | |||||
出版者別言語 | ||||||
Japanese Society for Dental Materials and Devices | ||||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 02874547 | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 18811361 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.4012/dmj.2012-188 | |||||
権利 | ||||||
権利情報 | © 2013 The Japanese Society for Dental Materials and Devices | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Dental Materials Journal, 32(2), pp.233-240; 2013 |