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Crystallization and preliminary crystallographic study of oligomers of the haemolytic lectin CEL-III from the sea cucumber Cucumaria echinata
http://hdl.handle.net/10069/32218
http://hdl.handle.net/10069/32218aa98e88a-fae1-4069-b153-c89dc449cc18
名前 / ファイル | ライセンス | アクション |
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ACSF_SBCC69_416.pdf (684.7 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2013-06-10 | |||||
タイトル | ||||||
タイトル | Crystallization and preliminary crystallographic study of oligomers of the haemolytic lectin CEL-III from the sea cucumber Cucumaria echinata | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Cucumaria echinata | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | haemolytic lectin CEL-III | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Unno, Hideaki
× Unno, Hideaki× Hisamatsu, Keigo× Nagao, Tomonao× Tateya, Yuki× Matsumoto, Naoki× Goda, Shuichiro× Hatakeyama, Tomomitsu |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | CEL-III is a Ca2+-dependent haemolytic lectin isolated from the marine invertebrate Cucumaria echinata. This lectin binds to Gal/GalNAc-containing carbohydrate chains on the cell surface and, after conformational changes, oligomerizes to form ion-permeable pores in cell membranes. CEL-III also forms soluble oligomers similar to those formed in cell membranes upon binding of specific carbohydrates in high-pH and high-salt solutions. These soluble and membrane CEL-III oligomers were crystallized and X-ray diffraction data were collected. Crystals of soluble oligomers and membrane oligomers diffracted X-rays to 3.3 and 4.2 Å resolution, respectively, using synchrotron radiation and the former was found to belong to space group C2. Self-rotation functional analysis of the soluble oligomer crystal suggested that it might be composed of heptameric CEL-III. | |||||
書誌情報 |
Acta Crystallographica Section F Structural Biology and Crystallization Communications 巻 69, 号 4, p. 416-420, 発行日 2013-04 |
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出版者 | ||||||
出版者 | International Union of Crystallography | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 17443091 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1107/S1744309113004065 | |||||
権利 | ||||||
権利情報 | © 2013 International Union of Crystallography | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Acta Crystallographica Section F Structural Biology and Crystallization Communications; 69(4), pp.416-420; 2013 |