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A New Approach to Establish a Cell Line with Reduced Risk of Endogenous Retroviruses
http://hdl.handle.net/10069/32385
http://hdl.handle.net/10069/32385bd69f6ee-28a4-4045-9591-e6d386663261
名前 / ファイル | ライセンス | アクション |
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PLoS8_61530.pdf (268.5 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2013-06-20 | |||||
タイトル | ||||||
タイトル | A New Approach to Establish a Cell Line with Reduced Risk of Endogenous Retroviruses | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Fukuma, Aiko
× Fukuma, Aiko× Yoshikawa, Rokusuke× Miyazawa, Takayuki× Yasuda, Jiro |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Endogenous retroviruses (ERVs) are integrated as DNA proviruses in the genomes of all mammalian species. Several ERVs are replication-competent and produced as fully infectious viruses from host cell. Thus, live-attenuated vaccines and biological substances have been prepared using the cell lines which may produce ERV. Indeed, we recently reported that several commercial live-attenuated vaccines for pets were contaminated with the infectious feline endogenous retrovirus, RD-114. In this study, to establish a cell line for vaccine manufacture with reduced risk of ERVs, we generated a cell line stably expressing human tetherin (Teth-CRFK cells). The release of infectious ERV from Teth-CRFK cells was suppressed to undetectable levels, while the production of parvovirus in Teth-CRFK cells was similar to that in parental CRFK cells. These observations suggest that Teth-CRFK cells will be useful as a cell line for the manufacture of live-attenuated vaccines or biological substances with reduced risk of ERV. | |||||
書誌情報 |
PLoS ONE 巻 8, 号 4, p. e61530, 発行日 2013-04-09 |
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出版者 | ||||||
出版者 | Public Library of Science | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 19326203 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1371/journal.pone.0061530 | |||||
権利 | ||||||
権利情報 | © 2013 Fukuma et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | PLoS ONE, 8(4), e61530; 2013 |