WEKO3
アイテム
{"_buckets": {"deposit": "e22c9b57-83cf-446c-8727-29cadb92d849"}, "_deposit": {"created_by": 2, "id": "765", "owners": [2], "pid": {"revision_id": 0, "type": "depid", "value": "765"}, "status": "published"}, "_oai": {"id": "oai:nagasaki-u.repo.nii.ac.jp:00000765", "sets": ["11"]}, "author_link": ["3579", "3580", "3578"], "item_2_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2020-03-05", "bibliographicIssueDateType": "Issued"}, "bibliographicPageStart": "122054", "bibliographicVolumeNumber": "1143", "bibliographic_titles": [{"bibliographic_title": "Journal of Chromatography B"}]}]}, "item_2_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "Glyoxylic acid (GA) is the intermediate metabolite in various mammalian metabolic pathways. GA showed high reactivity towards formation of advanced glycation end-products (AGEs); the main cause of pathogenesis and complications of many diseases. The presented study aimed to detect GA in healthy and cardiovascular patients’ (CV) sera; however analysis of GA in biological fluid is a challenge and requires chemical derivatization. Hence, a new, highly sensitive, time saving and reproducible pre-column fluorescence derivatization procedure coupled with high performance liquid chromatography (HPLC) method was developed. The derivatization method was based on reaction of 2-aminobenzenthiol (2-ABT), a fluorogenic reagent, with GA in acidic medium to form highly fluorescent thiazine derivative (290 and 390 nm for excitation and emission wavelengths respectively). The fluorescent derivative was separated within 6 min on a reversed-phase ODS column using an isocratic elution with a mixture of methanol?water (70:30, v/v%). The proposed method parameters were optimized and the method was validated. A good linearity in the concentration range (0.05?5.0 μM) was obtained with detection limit (LOD) of 10 nM (200 fmol/injection), which is more sensitive than several previous methods. Moreover, the recovery results were within the range of 85.0?95.5 % and the intra- and inter-day precision results were ?3.5%. It should be emphasized that this method is the first one for monitoring of GA in CV patients; to investigate its role for diagnosis and monitoring the severity and complications of this disease in clinical laboratory.", "subitem_description_type": "Abstract"}]}, "item_2_description_63": {"attribute_name": "引用", "attribute_value_mlt": [{"subitem_description": "Journal of Chromatography B, 1143, art.no.122054; 2020", "subitem_description_type": "Other"}]}, "item_2_publisher_33": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "Elsevier B.V."}]}, "item_2_relation_12": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type": "isVersionOf", "subitem_relation_type_id": {"subitem_relation_type_id_text": "10.1016/j.jchromb.2020.122054", "subitem_relation_type_select": "DOI"}}]}, "item_2_rights_13": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "c 2020 Elsevier B.V. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/"}]}, "item_2_source_id_7": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "15700232", "subitem_source_identifier_type": "ISSN"}]}, "item_2_version_type_16": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_ab4af688f83e57aa", "subitem_version_type": "AM"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Ali, Marwa F.B."}], "nameIdentifiers": [{"nameIdentifier": "3578", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kishikawa, Naoya"}], "nameIdentifiers": [{"nameIdentifier": "3579", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kuroda, Naotaka"}], "nameIdentifiers": [{"nameIdentifier": "3580", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2022-03-05"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "JChromB1143_122054.pdf", "filesize": [{"value": "450.7 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 450700.0, "url": {"label": "JChromB1143_122054.pdf", "url": "https://nagasaki-u.repo.nii.ac.jp/record/765/files/JChromB1143_122054.pdf"}, "version_id": "99728fc4-ac76-4cd5-a6f5-cb6321b51cca"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "Glyoxylic acid", "subitem_subject_scheme": "Other"}, {"subitem_subject": "2-Aminobenzenthiol", "subitem_subject_scheme": "Other"}, {"subitem_subject": "High Performance Liquid Chromatography(HPLC)", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Pre-column fluorescence derivatization", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Serum analysis", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Development of HPLC method for estimation of glyoxylic acid after pre-column fluorescence derivatization approach based on thiazine derivative formation: A new application in healthy and cardiovascular patients’ sera", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Development of HPLC method for estimation of glyoxylic acid after pre-column fluorescence derivatization approach based on thiazine derivative formation: A new application in healthy and cardiovascular patients’ sera"}]}, "item_type_id": "2", "owner": "2", "path": ["11"], "permalink_uri": "http://hdl.handle.net/10069/39949", "pubdate": {"attribute_name": "公開日", "attribute_value": "2020-12-18"}, "publish_date": "2020-12-18", "publish_status": "0", "recid": "765", "relation": {}, "relation_version_is_last": true, "title": ["Development of HPLC method for estimation of glyoxylic acid after pre-column fluorescence derivatization approach based on thiazine derivative formation: A new application in healthy and cardiovascular patients’ sera"], "weko_shared_id": 2}
Development of HPLC method for estimation of glyoxylic acid after pre-column fluorescence derivatization approach based on thiazine derivative formation: A new application in healthy and cardiovascular patients’ sera
http://hdl.handle.net/10069/39949
http://hdl.handle.net/10069/39949cccb9e9b-931f-4d24-b0ac-9d4f60d48e5f
名前 / ファイル | ライセンス | アクション |
---|---|---|
JChromB1143_122054.pdf (450.7 kB)
|
|
Item type | 学術雑誌論文 / Journal Article(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2020-12-18 | |||||
タイトル | ||||||
タイトル | Development of HPLC method for estimation of glyoxylic acid after pre-column fluorescence derivatization approach based on thiazine derivative formation: A new application in healthy and cardiovascular patients’ sera | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Glyoxylic acid | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 2-Aminobenzenthiol | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | High Performance Liquid Chromatography(HPLC) | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Pre-column fluorescence derivatization | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Serum analysis | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Ali, Marwa F.B.
× Ali, Marwa F.B.× Kishikawa, Naoya× Kuroda, Naotaka |
|||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Glyoxylic acid (GA) is the intermediate metabolite in various mammalian metabolic pathways. GA showed high reactivity towards formation of advanced glycation end-products (AGEs); the main cause of pathogenesis and complications of many diseases. The presented study aimed to detect GA in healthy and cardiovascular patients’ (CV) sera; however analysis of GA in biological fluid is a challenge and requires chemical derivatization. Hence, a new, highly sensitive, time saving and reproducible pre-column fluorescence derivatization procedure coupled with high performance liquid chromatography (HPLC) method was developed. The derivatization method was based on reaction of 2-aminobenzenthiol (2-ABT), a fluorogenic reagent, with GA in acidic medium to form highly fluorescent thiazine derivative (290 and 390 nm for excitation and emission wavelengths respectively). The fluorescent derivative was separated within 6 min on a reversed-phase ODS column using an isocratic elution with a mixture of methanol?water (70:30, v/v%). The proposed method parameters were optimized and the method was validated. A good linearity in the concentration range (0.05?5.0 μM) was obtained with detection limit (LOD) of 10 nM (200 fmol/injection), which is more sensitive than several previous methods. Moreover, the recovery results were within the range of 85.0?95.5 % and the intra- and inter-day precision results were ?3.5%. It should be emphasized that this method is the first one for monitoring of GA in CV patients; to investigate its role for diagnosis and monitoring the severity and complications of this disease in clinical laboratory. | |||||
書誌情報 |
Journal of Chromatography B 巻 1143, p. 122054, 発行日 2020-03-05 |
|||||
出版者 | ||||||
出版者 | Elsevier B.V. | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 15700232 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1016/j.jchromb.2020.122054 | |||||
権利 | ||||||
権利情報 | c 2020 Elsevier B.V. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Journal of Chromatography B, 1143, art.no.122054; 2020 |