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Interaction of Lipoplex with Albumin Enhances Gene Expression in Hepatitis Mice
http://hdl.handle.net/10069/39984
http://hdl.handle.net/10069/39984d53f18c9-282d-4cac-ba2e-ad1ee897f5e0
名前 / ファイル | ライセンス | アクション |
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Pharmaceutics12_341.pdf (1.5 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2020-05-20 | |||||
タイトル | ||||||
タイトル | Interaction of Lipoplex with Albumin Enhances Gene Expression in Hepatitis Mice | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Hepatitis | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | In vivo gene transfection | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Interaction with serum components | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Lipofection | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Liver | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Lung | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Yoshikawa, Naoki
× Yoshikawa, Naoki× Fumoto, Shintaro× Yoshikawa, Keiko× Hu, Die× Okami, Kazuya× Kato, Riku× Nakashima, Mikiro× Miyamoto, Hirotaka× Nishida, Koyo |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Understanding the in vivo fate of lipoplex, which is composed of cationic liposomes and DNA, is an important issue toward gene therapy. In disease conditions, the fate of lipoplex might change compared with the normal condition. Here, we examined the contribution of interaction with serum components to in vivo transfection using lipoplex in hepatitis mice. Prior to administration, lipoplex was incubated with serum or albumin. In the liver, the interaction with albumin enhanced gene expression in hepatitis mice, while in the lung, the interaction with serum or albumin enhanced it. In normal mice, the interaction with albumin did not enhance hepatic and pulmonary gene expression. Furthermore, hepatic and pulmonary gene expression levels of albumin-interacted lipoplex were correlated with serum transaminases in hepatitis mice. The albumin interaction increased the hepatic accumulation of lipoplex and serum tumor necrosis factor-α level. We suggest that the interaction with albumin enhanced the inflammation level after the administration of lipoplex in hepatitis mice. Consequently, the enhancement of the inflammation level might enhance the gene expression level. Information obtained in the current study will be valuable toward future clinical application of the lipoplex. | |||||
書誌情報 |
Pharmaceutics 巻 12, 号 4, p. 341, 発行日 2020-04-10 |
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出版者 | ||||||
出版者 | MDPI | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 19994923 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.3390/pharmaceutics12040341 | |||||
権利 | ||||||
権利情報 | c 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution(CC BY) license (http://creativecommons.org/licenses/by/4.0/). | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Pharmaceutics, 12(4), art.no.341; 2020 |