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A Simple and Rapid Determination of Alginate Lyase Activity
http://hdl.handle.net/10069/30761
http://hdl.handle.net/10069/307612eb7bd6f-2ccc-4437-b9b5-5ded66a8357a
名前 / ファイル | ライセンス | アクション |
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suisan40_035.pdf (211.7 kB)
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2013-02-21 | |||||
タイトル | ||||||
タイトル | A Simple and Rapid Determination of Alginate Lyase Activity | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
著者 |
Muramatsu, Tsuyoshi
× Muramatsu, Tsuyoshi |
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著者別名 | ||||||
姓名 | 村松, 毅 | |||||
その他のタイトル | ||||||
その他のタイトル | アルギン酸リアーゼの活性の簡易測定法 | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | A simple and rapid method for determination of alginate lyase activity has been investigated only by following the increase in absorbance in ultraviolet region of the reaction mixture. Partially purified enzyme from wreath shell (Turbo cornutus) acted on alginic acid, giving good linearity of the absorbance at 235 nm against the reaction time up to about 5min just after the initiation of the enzyme reaction under the condition applied. The initial reaction velocity can be easily obtained from the straight line in a simple operation. Alginate lyase 〔EC 4.2.99.4〕 acts on alginic acid, which is suggested to consist of mannuronic and guluronic acid, liberating uronides and uronic acid monomers with reducing end group and double bond on C4-C5 linkage of a pyranose ring at the site of the action. Based on the reducing power and the spectrophotometric or hydrodynamic properties of the reaction products, various methods for determination of alginate lyase activity have been reported. These methods are laborious, time-consuming and less useful for general kinetic analysis of enzyme action. In the experiments described in this paper, a simple and rapid method has been investigated only by following the increase in absorbance in ultraviolet region originated in formation of the double bond (eliminase action). Similar methods have been reported by Nakada et al., Preiss and Ashwell and Nakada and Sweeny for investigation of eliminase. | |||||
書誌情報 |
長崎大学水産学部研究報告 en : Bulletin of the Faculty of Fisheries, Nagasaki University 巻 40, p. 35-38, 発行日 1975-12 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 05471427 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AN00178473 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
論文ID(NAID) | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | NAID | |||||
関連識別子 | 40002770710 | |||||
出版者 | ||||||
出版者 | 長崎大学水産学部 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 長崎大学水産学部研究報告, v.40, pp.35-38; 1975 |