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Low-density Lipoprotein Receptor-related Protein-1 (LRP1) Mediates Autophagy and Apoptosis Caused by Helicobacter pylori VacA
http://hdl.handle.net/10069/31154
http://hdl.handle.net/10069/311547de690ce-8f53-414a-a168-e5e2b8b00cb9
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
JBC287_31104.pdf (1.3 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2013-04-01 | |||||
| タイトル | ||||||
| タイトル | Low-density Lipoprotein Receptor-related Protein-1 (LRP1) Mediates Autophagy and Apoptosis Caused by Helicobacter pylori VacA | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Autophagosomes | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Autophagy | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Caspase inhibitors | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Cell surface receptors | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Cellular damage | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Cytotoxins | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Gastric epithelial cells | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Helicobacter pylori | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Low density lipoproteins | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Mitochondrial damage | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 著者 |
Yahiro, Kinnosuke
× Yahiro, Kinnosuke× Satoh, Mamoru× Nakano, Masayuki× Hisatsune, Junzo× Isomoto, Hajime× Sap, Jan× Suzuki, Hidekazu× Nomura, Fumio× Noda, Masatoshi× Moss, Joel× Hirayama, Toshiya |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | In Helicobacter pylori infection, vacuolating cytotoxin (VacA)-induced mitochondrial damage leading to apoptosis is believed to be a major cause of cell death. It has also been proposed that VacA-induced autophagy serves as a host mechanism to limit toxin-induced cellular damage. Apoptosis and autophagy are two dynamic and opposing processes that must be balanced to regulate cell death and survival. Here we identify the low-density lipoprotein receptor-related protein-1 (LRP1) as the VacA receptor for toxin-induced autophagy in the gastric epithelial cell line AZ-521, and show that VacA internalization through binding to LRP1 regulates the autophagic process including generation of LC3-II from LC3-I, which is involved in formation of autophagosomes and autolysosomes. Knockdown of LRP1 and Atg5 inhibited generation of LC3-II as well as cleavage of PARP, a marker of apoptosis, in response to VacA, whereas caspase inhibitor, benzyloxycarbonyl-VAD- fluoromethylketone (Z-VAD-fmk), and necroptosis inhibitor, Necrostatin-1, did not inhibit VacA-induced autophagy, suggesting that VacA-induced autophagy via LRP1 binding precedes apoptosis. Other VacA receptors such as RPTPα, RPTPβ, and fibronectin did not affect VacA-induced autophagy or apoptosis. Therefore, we propose that the cell surface receptor, LRP1, mediates VacA-induced autophagy and apoptosis. | |||||
| 書誌情報 |
Journal of Biological Chemistry 巻 287, 号 37, p. 31104-31115, 発行日 2012-09-07 |
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| 出版者 | ||||||
| 出版者 | American Society for Biochemistry and Molecular Biology Inc. | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 00219258 | |||||
| EISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 1083351X | |||||
| DOI | ||||||
| 関連タイプ | isVersionOf | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1074/jbc.M112.387498 | |||||
| 権利 | ||||||
| 権利情報 | © 2012 by The American Society for Biochemistry and Molecular Biology, Inc. | |||||
| 権利 | ||||||
| 権利情報 | This research was originally published in Journal of Biological Chemistry. Kinnosuke Yahiro, Mamoru Satoh, Masayuki Nakano, Junzo Hisatsune, Hajime Isomoto, Jan Sap, Hidekazu Suzuki, Fumio Nomura, Masatoshi Noda, Joel Moss and Toshiya Hirayama. Low-density Lipoprotein Receptor-related Protein-1 (LRP1) Mediates Autophagy and Apoptosis Caused by Helicobacter pylori VacA. Journal of Biological Chemistry. 2012; 287(37):pp.31104-31115. the American Society for Biochemistry and Molecular Biology. | |||||
| 著者版フラグ | ||||||
| 出版タイプ | AM | |||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
| 引用 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Journal of Biological Chemistry, 287(37), pp.31104-31115; 2012 | |||||
